PXD048361

PXD048361 is an original dataset announced via ProteomeXchange.

Title	Immunomodulatory, antioxidant, and potential anticancer activity of the polysaccharides of the fungus Fomitiporia chilensis
Description	Fomitiporia species have aroused the interest of numerous investigations that reveal their biological activity and medicinal potential. The present investigation shows the antioxi-dant, anticancer, and immunomodulatory activity of acidic polysaccharides obtained from the fungus Fomitiporia chilensis. The acidic polysaccharides were obtained for acidic precipitation with 2% O-N-cetyl pyridinium bromide. Chemical analysis was performed by FT-IR and GC-MS methods. The antioxidant capacity of acidic polysaccharides from F. chilensis was evaluated by the scavenging of free radicals with the ABTS and DPPH as-says. Macrophages proliferation and cytokine production assays were used to determine the immunomodulatory capacity of the polysaccharides. Anti-tumor and cytotoxicity ac-tivity was evaluated with MTT assay in the U-937, HTC-116, and HGF-1 cell lines. The ef-fect of polysaccharides on the cell cycle of the HCT-116 cell line was determined for flow cytometry. Fourier Transform-infrared characterization revealed characteristic absorption peaks for polysaccharides, whereas the GC-MS analysis detected three peak correspond-ing to D-galactose, galacturonic acid and D-glucose. The TNF-α proliferation was in-creased when the cell was treated with 2 mg/mL polysaccharides, whereas the IL-6 con-centration was increased with all polysaccharide concentrations evaluated. The cell cycle analysis of HTC-116 treated with polysaccharides evidenced that the acidic polysaccha-rides from F. chilensis induce an increase in the G0/G1 cell cycle phase, incrementing the apoptotic cell percentage. A proteomic analysis suggested some of the molecular mecha-nisms involved in their antioxidant and cellular detoxifying effects and justified their tra-ditional use in heart diseases.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:56:27.219.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Casimiro Cardenas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-09 13:30:29	ID requested
⏵ 1	2024-10-17 05:56:28	announced

10.3390/molecules29153628;

Abdala-D, í, az RT, Casas-Arrojo V, Castro-Varela P, Riquelme C, Carrillo P, Medina M Á, C, á, rdenas C, Becerra J, P, é, rez Manr, í, quez C, . Molecules, 29(15):(2024) [pubmed]

submitter keyword: Fomitiporia chilensis, HGF-1 cells, UHPLC-HRMS, polysaccharides

Roberto Abdala Díaz
contact affiliation	Malaga University, Institute of Blue Biotechnology and Development (IBYDA), Department of Ecology and Geology, Malaga, Spain
contact email	abdala@uma.es
lab head
Casimiro Cardenas
contact affiliation	University of Malaga
contact email	ccg@uma.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048361
     1. Label: PRIDE project
     2. Name: Immunomodulatory, antioxidant, and potential anticancer activity of the polysaccharides of the fungus Fomitiporia chilensis