PXD048330

PXD048330 is an original dataset announced via ProteomeXchange.

Title	Sty1 kinase-dependent phosphosite mapping on Atf1
Description	In the fission yeast Schizosaccharomyces pombe, heterochromatic silencing is quite stable at pericentromeres but unstable at the mating-type (mat) locus under chronic heat stress. We found that the compromised gene silencing at the mat locus at elevated temperature is linked to the phosphorylation status of Atf1 (a member of the ATF/CREB superfamily) by Sty1, one of the mitogen-activated protein kinases (MAPKs). To gain a full picture of Sty1-dependent phosphosite map of Atf1 under heat stress, we purified HA-Atf1 protein from cultures of wild type and sty1Δ strains shifted from 25℃ to either 30℃ or 37℃ for 5 hr followed by immunoprecipitation. Samples were separated by SDS-PAGE, and Coomassie blue-stained gel slices were excised and submitted for mass spectrometry analyses.
HostingRepository	PRIDE
AnnounceDate	2024-02-15
AnnouncementXML	Submission_2024-02-15_03:42:55.952.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Quan-wen Jin
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	phosphorylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-08 22:57:07	ID requested
⏵ 1	2024-02-15 03:42:56	announced

10.7554/ELIFE.90525;

submitter keyword: Atf1，phosphorylation，Sty1，fission yeast (Schizosaccharomyces pombe)

Quan-wen Jin
contact affiliation	State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian, China
contact email	jinquanwen@xmu.edu.cn
lab head
Quan-wen Jin
contact affiliation	School of Life Sciences, Xiamen University
contact email	jinquanwen@xmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048330
     1. Label: PRIDE project
     2. Name: Sty1 kinase-dependent phosphosite mapping on Atf1