PXD048310

PXD048310 is an original dataset announced via ProteomeXchange.

Title	ChAHP and ChAHP2 control diverse retrotransposons by complementary activities.
Description	Retrotransposon control in mammals is an intricate process that is effectuated by a broad network of chromatin regulatory pathways. We previously discovered ChAHP, a protein complex with repressive activity against SINE retrotransposons, composed of the transcription factor ADNP, chromatin remodeler CHD4, and HP1 proteins. Here we identify ChAHP2, a protein complex homologous to ChAHP, wherein ADNP is replaced by ADNP2. ChAHP2 is predominantly targeted to ERVs and LINEs, via HP1-mediated binding of H3K9 trimethylated histones. We further demonstrate that ChAHP also binds these elements in a mechanistically equivalent manner to ChAHP2, and distinct from DNA sequence-specific recruitment at SINEs. Genetic ablation of ADNP2 alleviates ERV and LINE1 repression, which is synthetically exacerbated by additional depletion of ADNP. Together, our results reveal that the ChAHP and ChAHP2 complexes function to control both non-autonomous and autonomous retrotransposons by complementary activities, further adding to the complexity of mammalian transposon control.
HostingRepository	PRIDE
AnnounceDate	2025-08-05
AnnouncementXML	Submission_2025-08-05_03:30:17.941.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Hess
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-08 07:14:30	ID requested
⏵ 1	2025-08-05 03:30:18	announced

10.1101/GAD.351769.124;

submitter keyword: ADNP2, mouse ESC, HP1, CHD4, retrotransposon control,ADNP

Marc Buehler
contact affiliation	Department of Genome Regulation Friedrich Miescher Institute for Biomedical Research Maulbeerstr. 66 4058 Basel Switzerland
contact email	marc.buehler@fmi.ch
lab head
Daniel Hess
contact affiliation	Friedrich Miescher Institute for Biomedical Research
contact email	dhess@fmi.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048310
     1. Label: PRIDE project
     2. Name: ChAHP and ChAHP2 control diverse retrotransposons by complementary activities.