PXD048296

PXD048296 is an original dataset announced via ProteomeXchange.

Title	Enhanced Branched-Chain Amino Acid Metabolism Improves 7 Age-Related Reproduction
Description	Reproductive aging is one of the earliest human aging phenotypes, and mitochondrial dysfunction has been linked to a decline in oocyte quality. However, it is not known which mitochondrial metabolic processes are critical for oocyte quality maintenance with age. To understand how mitochondrial processes contribute to C. elegans oocyte quality, we characterized the mitochondrial proteomes of young and aged wild-type and long-reproductive daf-2 mutants. The mitochondrial proteomic profiles of young wild-type and daf-2 worms are similar and share upregulation of branched-chain amino acid (BCAA) metabolism pathway enzymes. Reduction of bcat-1 shortens reproduction, elevates mitochondrial ROS levels, and shifts mitochondrial localization. Moreover, bcat-1 knockdown decreases daf-2’s oocyte quality and reduces reproductive capability, indicating the importance of this pathway in the maintenance of oocyte quality with age. Importantly, we can delay oocyte quality deterioration and extend reproduction in wild-type animals both by bcat-1 overexpression and by supplementing with Vitamin B1, a cofactor needed for BCAA metabolism.
HostingRepository	PRIDE
AnnounceDate	2024-01-15
AnnouncementXML	Submission_2024-01-15_10:02:05.724.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yishai Levin
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-07 05:29:37	ID requested
⏵ 1	2024-01-15 10:02:06	announced

Dataset with its publication pending

submitter keyword: Reproductive, mytochondria, proteomics, aging

Coleen T. 13 Murphy
contact affiliation	1Department of Molecular Biology & 2LSI Genomics, Princeton University
contact email	ctmurphy@princeton.edu
lab head
Yishai Levin
contact affiliation	Weizmann Institute of Science, Israel
contact email	yishai.levin@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD048296

PRIDE project URI

• PRIDE
  1. PXD048296
     1. Label: PRIDE project
     2. Name: Enhanced Branched-Chain Amino Acid Metabolism Improves 7 Age-Related Reproduction