PXD048293

PXD048293 is an original dataset announced via ProteomeXchange.

Title	Cyclic ion mobility for hydrogen/deuterium exchange-mass spectrometry applications
Description	Hydrogen/deuterium exchange-mass spectrometry (HDX-MS) has emerged as a powerful tool to probe protein dynamics. As a bottom-up technique, HDX-MS provides information at peptide-level resolution, allowing structural localisation of dynamic changes. Consequently, HDX-MS data quality is largely determined by the number of peptides that are identified and monitored after deuteration. Integration of ion mobility (IM) into HDX-MS workflows has been shown to increase data quality by providing an orthogonal mode of peptide ion separation in the gas-phase. This is of critical importance for challenging targets such as integral membrane proteins (IMPs), which often suffer from low sequence coverage and/or redundancy in HDX-MS analyses. The increasing complexity of samples being investigated by HDX-MS, such as membrane mimetic reconstituted and in vivo IMPs, has generated need for instrumentation with greater resolving power. Recently, Giles et al. developed cyclic ion mobility (cIM), an IM device with racetrack geometry that enables scalable, multi-pass IM separations. Using 1-pass and multi-pass cIM routines, we use the recently commercialised SELECT SERIES™ Cyclic™ IM spectrometer for HDX-MS analyses of 4 detergent solubilised IMP samples and report its enhanced performance. Furthermore, we develop a novel processing strategy capable of better handling multi-pass cIM data. Interestingly, use of 1-pass and multi-pass cIM routines produced unique peptide populations, with their combined peptide output being 31 to 222% higher than previous generation SYNAPT G2-Si instrumentation. Thus, we propose a novel HDX-MS workflow with integrated cIM which has the potential to enable the analysis of more complex systems with greater accuracy and speed.
HostingRepository	PRIDE
AnnounceDate	2024-04-08
AnnouncementXML	Submission_2024-04-08_07:33:54.948.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Damon Griffiths
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS; SELECT SERIES Cyclic IMS

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-06 22:42:00	ID requested
⏵ 1	2024-04-08 07:33:55	announced

10.1021/ACS.ANALCHEM.3C05753;

submitter keyword: mass spectrometry, cyclic ion mobility, membrane proteins, HDX-MS,Hydrogen/deuteirum exchange

Argyris Politis
contact affiliation	The University of Manchester
contact email	argyris.politis@manchester.ac.uk
lab head
Damon Griffiths
contact affiliation	The University of Manchester
contact email	damon.griffiths@postgrad.manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD048293

PRIDE project URI

• PRIDE
  1. PXD048293
     1. Label: PRIDE project
     2. Name: Cyclic ion mobility for hydrogen/deuterium exchange-mass spectrometry applications