PXD048279

PXD048279 is an original dataset announced via ProteomeXchange.

Title	Leaf proteome responses to water deficit and sulfur deficiency in pea (Pisum sativum)
Description	Combination of stresses often induce specific molecular responses in plants, which could not be predicted from the effects of the same stresses applied individually. Sulfur availability in soils affects both yield and seed quality in major crops, and the plant capacity to tolerate other environmental constraints. To dissect the molecular responses to sulfur deficiency, and how water deficit alters the effects, we employed a multi-omics approach in leaves of Pisum sativum, in which both yield and seed quality are compromised by these two stressors. Using transcriptomics, proteomics and gene co-expression network analyses, we identified a module of genes strongly driven by S availability. This includes known and putative new players of plant responses to S-deprived conditions. Conserved profiles between proteins and mRNAs were specifically observed within this module, suggesting transcriptional regulation. Under stress combination, plant growth was severely compromised, and molecular responses were amplified, notably at the transcriptome level, in a time-specific manner. Transcriptional regulators of the highlighted genes and pathways were predicted, which may represent interesting targets to develop crops tolerant to multi-stress conditions. The produced datasets also represent important resources for our knowledge of the plant molecular responses specifically induced under single or combined stresses in plants.
HostingRepository	PRIDE
AnnounceDate	2026-09-01
AnnouncementXML	Submission_2026-09-01_09:37:50.560.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thierry Balliau
SpeciesList	scientific name: Pisum sativum (Garden pea); NCBI TaxID: NEWT:3888;
ModificationList	(R)-5-oxo-1; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-05 09:26:20	ID requested
⏵ 1	2026-09-01 09:37:51	announced

Bonnot T, Henriet C, Aim, é D, Kreplak J, T, é, r, é, zol M, Rossin N, Balliau T, Blanchard C, Lamotte O, Ourry A, Zivy M, Vernoud V, Gallardo-Guerrero K, Combined sulfur deficiency and water deficit trigger synergistic redox adjustments through coordinated transcript-protein regulation in pea. J Exp Bot, ():(2026) [pubmed]

10.1093/jxb/erag219;

submitter keyword: abiotic stresses, leaf,Pea, proteome

Titouan Bonnot
contact affiliation	Agroécologie, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France
contact email	titouan.bonnot@inrae.fr
lab head
Thierry Balliau
contact affiliation	Plateforme d'Analyse Proteomique Paris Sud-Ouest Université Paris-Saclay, INRAE, CNRS, AgroParisTech, GQE - Le Moulon, 91190, Gif-sur-Yvette, France
contact email	thierry.balliau@inrae.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD048279

PRIDE project URI

• PRIDE
  1. PXD048279
     1. Label: PRIDE project
     2. Name: Leaf proteome responses to water deficit and sulfur deficiency in pea (Pisum sativum)