PXD048267

PXD048267 is an original dataset announced via ProteomeXchange.

Title	Proteome labeled by a cuprous ion-responsive protein labeling reagent in the ATP7A WT and ATP7A KO living cells
Description	We have developed a new molecular probe called CuR. This probe is selectively activated by labile Cu(I), hence enabling labeling of the proteins in Cu(I)-rich cellular environments. We applied CuR to ATP7A-WT and ATP7A-KO cells that were pretreated with or without Cu(gtsm). Next, we enriched the labeled proteins by immunoprecipitation, digested them with trypsin, and analyzed them using liquid chromatography-tandem mass spectrometry. We used label-free quantification in the mass spectrometry analysis to detect changes in labeled proteins caused by exogenous copper compared to the non-copper-treated condition.
HostingRepository	jPOST
AnnounceDate	2025-01-05
AnnouncementXML	Submission_2025-01-04_07:00:03.971.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rong Cheng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-05 00:51:34	ID requested
⏵ 1	2025-01-04 07:00:04	announced

Dataset with its publication pending

submitter keyword: Copper ions, Chemical proteomics, Cu(I)-responsive, ATP7A

Itaru Hamachi
lab head
Rong Cheng
contact affiliation	Kyoto University
dataset submitter

jPOST dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002440/