PXD048255 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | GroEL-proteotypbing-based stable isotope probing of Thauera aromatica K172, Pseudomonas putida KT2440, and Escherichia coli BL21(DE3) |
Description | This dataset has been used to establish GroEL-SIP, coupling a targeted proteotyping approach for assessing bacterial community compositions using the taxonomic marker protein GroEL with stable isotope probing to link the identified taxa to substrate assimilation. This dataset contains raw data of four experiments: 1.) Pure cultures of T. aromatica cultivated with 13C or 12C benzoate mixed in defined ratios. 2.) Pure cultures of P. putida cultivated with 13C or 12C benzoate mixed in a 1:1 ratio. 3.) Pure cultures of E. coli cultivated with 12C acetate and 13C or 12C benzoate mixed in a 1:1 ratio. 4.) Co-cultivated biculture of T. aromatica and P. putida cultivated with 13C or 12C benzoate and mixed in a 1:1 ratio. 5.) Co-cultivated biculture of T. aromatica and E. coli cultivated with 12c acetate and 13C or 12C benzoate and mixed in a 1:1 ratio. 1)-3) were analyzed after in-solution digestion 4)-5) were analyzed after in-gel digestion of the 60 kDa band |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_08:19:31.500.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Simon Klaes |
SpeciesList | scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Thauera aromatica K172; NCBI TaxID: 44139; scientific name: Escherichia coli BL21(DE3); NCBI TaxID: 469008; scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-04 09:51:34 | ID requested | |
⏵ 1 | 2024-10-17 08:19:31 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: protein-SIP, stable isotope probing,GroEL-proteotyping |
Contact List
Lorenz Adrian |
contact affiliation | Helmholtz Centre for Environmental Research - UFZ, Environmental Biotechnology and Technische Universität Berlin, Geobiotechnology |
contact email | lorenz.adrian@ufz.de |
lab head | |
Simon Klaes |
contact affiliation | Helmholtz Centre for Environmental Research - UFZ |
contact email | simon.klaes@ufz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048255
- Label: PRIDE project
- Name: GroEL-proteotypbing-based stable isotope probing of Thauera aromatica K172, Pseudomonas putida KT2440, and Escherichia coli BL21(DE3)