PXD048244

PXD048244 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Lactylation of nucleus pulposus cells
Description	Nucleus pulposus (NP) resides in hypoxic microenvironment due to the avascular structure of intervertebral disc (IVD). Intracellular lactate drives lysine lactylation (Kla) as a newly epigenetic modification. However, the impact of Kla on NPCs remains unknown. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we showed a global lactylome profiling on NPCs cultured in normoxia and hypoxia environment and found that 3510 lactylation sites on 1052 proteins in NPCs on non-histone proteins. Moreover, there are 18 proteins with 129 Kla sites exclusively detected in the normoxia group, and 117 Kla sites in 27 proteins were specific in hypoxia group. Together, our dates reveals that Kla plays an important role in regulating cellular metabolism and may contribute to IDD progression.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_12:42:18.470.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jun Dai
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue
Instrument	autoflex

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-03 23:20:03	ID requested
⏵ 1	2025-05-06 12:42:19	announced

Publication List

Sheng L, Xu H, Wang Y, Ni J, Xiang T, Xu H, Zhou X, Wei K, Dai J, Systematic analysis of lysine lactylation in nucleus pulposus cells. iScience, 27(11):111157(2024) [pubmed]
10.1016/j.isci.2024.111157;

Sheng L, Xu H, Wang Y, Ni J, Xiang T, Xu H, Zhou X, Wei K, Dai J, Systematic analysis of lysine lactylation in nucleus pulposus cells. iScience, 27(11):111157(2024) [pubmed]

10.1016/j.isci.2024.111157;

Keyword List

submitter keyword: lactylation,nucleus pulposus cells, intervertebral disc degeneration

submitter keyword: lactylation,nucleus pulposus cells, intervertebral disc degeneration

Contact List

Jun Dai
contact affiliation	The Second Affiliated Hospital of Soochow University
contact email	daijun@suda.edu.cn
lab head
Jun Dai
contact affiliation	The Second Affiliated Hospital of Soochow University
contact email	daijun@suda.edu.cn
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD048244
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD048244

PRIDE project URI

Repository Record List

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