PXD048233
PXD048233 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Interaction proteomics of cortical GABAA receptor subunits identifies novel interactors |
| Description | GABAA receptors (GABAAR) are pentameric ligand-gated ion channels responsible for most of the inhibitory synaptic transmission in the brain. The distinct physiological and pharmacological properties of these receptors are underlain by unique subunit composition and receptor associated proteins, where the most abundant form of synaptic GABAAR comprises of 2 copies of α1, 2 copies of β, and one copy of γ2 subunits. To reveal novel receptor protein complexes, we performed affinity-based proteomics analysis from mouse cortex extract using antibodies against GABAAR α1, β2/3, and γ2 subunits, as well as antibodies against the known GABAAR interacting proteins Nlgn2. From this analysis various interactions between GABAAR α subunits were identified. Focusing on the intersection of the three subunit proteomes yielded a more precise proteome analysis of a specific GABAAR subtype, and revealed Camkv and Gpc as novel GABAAR interactors. Their direct interaction was validated by co-expression and co-immunoprecipitation experiments. |
| HostingRepository | iProX |
| AnnounceDate | 2024-01-04 |
| AnnouncementXML | Submission_2024-01-03_18:53:45.134.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Smores |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-01-03 18:53:30 | ID requested | |
| ⏵ 1 | 2024-01-03 18:53:45 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: GABAAR, immunoprecipitation, proteomics, mass spectrometry |
Contact List
| Kawan Li | |
|---|---|
| contact affiliation | Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam |
| contact email | k.w.li@vu.nl |
| lab head | |
| Smores | |
| contact affiliation | Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam |
| contact email | smores1990@hotmail.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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