PXD048229

PXD048229 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Molecular basis of mRNA delivery to the bacterial ribosome by bS1 protein and RNA polymerase
Description	The initiation of translation begins with formation of a ribosome-mRNA complex. In bacteria, the 30S ribosomal subunit is recruited to many mRNAs through both base pairing with the Shine Dalgarno (SD) sequence and RNA-binding by ribosomal protein bS1. Translation can initiate on mRNAs that are being transcribed, and RNA polymerase (RNAP) can promote recruitment of the pioneering 30S. Here we have examined ribosome recruitment to mRNAs using cryogenic electron microscopy (cryo-EM), single-molecule fluorescence co-localization, and whole-cell cross-linking mass spectrometry. Structures of 30S-mRNA complexes show that bS1 delivers the mRNA to the ribosome for SD duplex formation and initial 30S subunit activation. RNAP associates flexibly with the 30S platform during nascent mRNA delivery and accelerates their association in a bS1-dependent manner in vitro. Collectively, our data provide a mechanistic framework for how the SD duplex, ribosomal proteins and RNAP cooperate in 30S recruitment to mRNAs and thereby establish transcription-translation coupling.
HostingRepository	jPOST
AnnounceDate	2024-11-15
AnnouncementXML	Submission_2024-11-14_23:20:15.189.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048229
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Graziadei
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; unknown modification; unknown modification; unknown modification; unknown modification
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-03 14:21:45	ID requested
⏵ 1	2024-11-14 23:20:15	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: crosslinking mass spectrometry, escherichia coli, RNA polymerase, ribosome

submitter keyword: crosslinking mass spectrometry, escherichia coli, RNA polymerase, ribosome

Contact List

Juri Rappsilber
lab head
Andrea Graziadei
contact affiliation	Fondazione Human Technopole
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002435/