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PXD048228

PXD048228 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleApplying Proteomics and Computational Approaches to Identify Novel Targets in Blast Associated Post-Traumatic Epilepsy
DescriptionTraumatic brain injury (TBI) remains a leading cause of post traumatic epilepsy (PTE). Blast TBI (bTBI) found in Veterans presents cognitive and behavioral disturbance such as PTE, however, the underlying mechanisms that drive the long-term sequelae are not well understood. Using an unbiased proteomics approach in a murine model of repeated bTBI (rbTBI), this study addresses this gap in knowledge. Following rbTBI, mice underwent 24/7 EEG recording for four months before collecting the cortex and hippocampus of PTE+, PTE- and sham mice. Hundreds of differentially expressed proteins were identified in the cortex and hippocampus of PTE+ and PTE- relative to sham. Focusing on protein pathways unique to PTE+ animals revealed mitochondrial function, post translational modifications, and protein transport proteins were disrupted. Computational metabolic modeling using dysregulated protein expression predicted mitochondrial proton pump dysregulation, suggesting electron transport chain dysregulation in epileptic tissue relative to PTE- and sham. Finally, data mining enabled the identification of several novel and previously validated TBI and epilepsy biomarkers in our data set, many of which were found to already be targeted by drugs in various phases of clinical testing. These findings highlight novel proteins and protein pathways that may drive the chronic PTE sequala following rbTBI.
HostingRepositoryPRIDE
AnnounceDate2024-06-22
AnnouncementXMLSubmission_2024-06-22_10:03:38.819.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD048228
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKeith Ray
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-03 14:02:14ID requested
12024-06-22 10:03:39announced
Publication List
Browning JL, Wilson KA, Shandra O, Wei X, Mahmutovic D, Maharathi B, Robel S, VandeVord PJ, Olsen ML, Applying Proteomics and Computational Approaches to Identify Novel Targets in Blast-Associated Post-Traumatic Epilepsy. Int J Mol Sci, 25(5):(2024) [pubmed]
10.6019/PXD048228;
10.3390/ijms25052880;
Keyword List
submitter keyword: concussive brain injury, blast neurotrauma,traumatic brain injury, post traumatic epilepsy
Contact List
Michelle L. Olsen
contact affiliationProfessor and Director, School of Neuroscience, Virginia Tech., Blacksburg, VA, USA
contact emailmolsen1@vt.edu
lab head
Keith Ray
contact affiliationVirginia Tech
contact emailwkray@vt.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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