PXD048228

PXD048228 is an original dataset announced via ProteomeXchange.

Title	Applying Proteomics and Computational Approaches to Identify Novel Targets in Blast Associated Post-Traumatic Epilepsy
Description	Traumatic brain injury (TBI) remains a leading cause of post traumatic epilepsy (PTE). Blast TBI (bTBI) found in Veterans presents cognitive and behavioral disturbance such as PTE, however, the underlying mechanisms that drive the long-term sequelae are not well understood. Using an unbiased proteomics approach in a murine model of repeated bTBI (rbTBI), this study addresses this gap in knowledge. Following rbTBI, mice underwent 24/7 EEG recording for four months before collecting the cortex and hippocampus of PTE+, PTE- and sham mice. Hundreds of differentially expressed proteins were identified in the cortex and hippocampus of PTE+ and PTE- relative to sham. Focusing on protein pathways unique to PTE+ animals revealed mitochondrial function, post translational modifications, and protein transport proteins were disrupted. Computational metabolic modeling using dysregulated protein expression predicted mitochondrial proton pump dysregulation, suggesting electron transport chain dysregulation in epileptic tissue relative to PTE- and sham. Finally, data mining enabled the identification of several novel and previously validated TBI and epilepsy biomarkers in our data set, many of which were found to already be targeted by drugs in various phases of clinical testing. These findings highlight novel proteins and protein pathways that may drive the chronic PTE sequala following rbTBI.
HostingRepository	PRIDE
AnnounceDate	2024-06-22
AnnouncementXML	Submission_2024-06-22_10:03:38.819.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048228
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Keith Ray
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-03 14:02:14	ID requested
⏵ 1	2024-06-22 10:03:39	announced

Browning JL, Wilson KA, Shandra O, Wei X, Mahmutovic D, Maharathi B, Robel S, VandeVord PJ, Olsen ML, Applying Proteomics and Computational Approaches to Identify Novel Targets in Blast-Associated Post-Traumatic Epilepsy. Int J Mol Sci, 25(5):(2024) [pubmed]

10.6019/PXD048228;

10.3390/ijms25052880;

submitter keyword: concussive brain injury, blast neurotrauma,traumatic brain injury, post traumatic epilepsy

Michelle L. Olsen
contact affiliation	Professor and Director, School of Neuroscience, Virginia Tech., Blacksburg, VA, USA
contact email	molsen1@vt.edu
lab head
Keith Ray
contact affiliation	Virginia Tech
contact email	wkray@vt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD048228

PRIDE project URI

• PRIDE
  1. PXD048228
     1. Label: PRIDE project
     2. Name: Applying Proteomics and Computational Approaches to Identify Novel Targets in Blast Associated Post-Traumatic Epilepsy