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PXD048224

PXD048224 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteolysis pattern of two variants of Chickpea using LC-MS/MS
DescriptionDespite the importance of grains and legumes in the human diet, little is known regarding peptide release and the temporal changes of protease activities during seed germination. LC/MS-MS peptidomic analysis of two cultivars of germinating chickpea followed by computational analyses indicated cleavage dominated by proteases with a single position preference (mainly before (P1) or after cleavage (P1’): L at P2 (cysEP-like); R or K at P1 (vignain-like), N or Q at P1 (legumain-like); and previously unidentified K, R, A and S at P1’; A at P2’). While P1 N cleavages were relatively constant, P1’ K/R preferences were high in soaked garbanzo (kabuli) seeds, declined by four days, and returned at six days, but were much rarer in the brown (desi) cultivar. Late Embryogenesis Associated (LEA) peptides weremarkedly released during early germination. Vicilin peptides rich in glutamic acid near their N-termini markedly increased with germination, consistent with strong proteolytic resistance, even to human digestion, as indicated by analyses of separate datasets. Thus, this first peptidomics study of seed germination proteolytic profiles unveils a complex cultivar-specific programme of sequential activation and inactivation of a series of proteases, associated with the differential release of peptides from different protein groups.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:50:46.761.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIndrani Bera
SpeciesList scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-03 07:31:13ID requested
12024-07-18 08:25:28announced
22024-10-22 06:50:47announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: peptidomics,Proteolysis pattern, plant seed proteins
Contact List
DENIS SHIELDS
contact affiliationSchool of Medicine, UCD, Dublin, Ireland
contact emaildenis.shields@ucd.ie
lab head
Indrani Bera
contact affiliationUCD Dublin
contact emailindrani.bera@ucd.ie
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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