PXD048224

PXD048224 is an original dataset announced via ProteomeXchange.

Title	Proteolysis pattern of two variants of Chickpea using LC-MS/MS
Description	Despite the importance of grains and legumes in the human diet, little is known regarding peptide release and the temporal changes of protease activities during seed germination. LC/MS-MS peptidomic analysis of two cultivars of germinating chickpea followed by computational analyses indicated cleavage dominated by proteases with a single position preference (mainly before (P1) or after cleavage (P1’): L at P2 (cysEP-like); R or K at P1 (vignain-like), N or Q at P1 (legumain-like); and previously unidentified K, R, A and S at P1’; A at P2’). While P1 N cleavages were relatively constant, P1’ K/R preferences were high in soaked garbanzo (kabuli) seeds, declined by four days, and returned at six days, but were much rarer in the brown (desi) cultivar. Late Embryogenesis Associated (LEA) peptides weremarkedly released during early germination. Vicilin peptides rich in glutamic acid near their N-termini markedly increased with germination, consistent with strong proteolytic resistance, even to human digestion, as indicated by analyses of separate datasets. Thus, this first peptidomics study of seed germination proteolytic profiles unveils a complex cultivar-specific programme of sequential activation and inactivation of a series of proteases, associated with the differential release of peptides from different protein groups.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:50:46.761.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Indrani Bera
SpeciesList	scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-03 07:31:13	ID requested
1	2024-07-18 08:25:28	announced
⏵ 2	2024-10-22 06:50:47	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: peptidomics,Proteolysis pattern, plant seed proteins

DENIS SHIELDS
contact affiliation	School of Medicine, UCD, Dublin, Ireland
contact email	denis.shields@ucd.ie
lab head
Indrani Bera
contact affiliation	UCD Dublin
contact email	indrani.bera@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD048224

PRIDE project URI

• PRIDE
  1. PXD048224
     1. Label: PRIDE project
     2. Name: Proteolysis pattern of two variants of Chickpea using LC-MS/MS