PXD048224 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteolysis pattern of two variants of Chickpea using LC-MS/MS |
Description | Despite the importance of grains and legumes in the human diet, little is known regarding peptide release and the temporal changes of protease activities during seed germination. LC/MS-MS peptidomic analysis of two cultivars of germinating chickpea followed by computational analyses indicated cleavage dominated by proteases with a single position preference (mainly before (P1) or after cleavage (P1’): L at P2 (cysEP-like); R or K at P1 (vignain-like), N or Q at P1 (legumain-like); and previously unidentified K, R, A and S at P1’; A at P2’). While P1 N cleavages were relatively constant, P1’ K/R preferences were high in soaked garbanzo (kabuli) seeds, declined by four days, and returned at six days, but were much rarer in the brown (desi) cultivar. Late Embryogenesis Associated (LEA) peptides weremarkedly released during early germination. Vicilin peptides rich in glutamic acid near their N-termini markedly increased with germination, consistent with strong proteolytic resistance, even to human digestion, as indicated by analyses of separate datasets. Thus, this first peptidomics study of seed germination proteolytic profiles unveils a complex cultivar-specific programme of sequential activation and inactivation of a series of proteases, associated with the differential release of peptides from different protein groups. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:50:46.761.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Indrani Bera |
SpeciesList | scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-03 07:31:13 | ID requested | |
1 | 2024-07-18 08:25:28 | announced | |
⏵ 2 | 2024-10-22 06:50:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: peptidomics,Proteolysis pattern, plant seed proteins |
Contact List
DENIS SHIELDS |
contact affiliation | School of Medicine, UCD, Dublin, Ireland |
contact email | denis.shields@ucd.ie |
lab head | |
Indrani Bera |
contact affiliation | UCD Dublin |
contact email | indrani.bera@ucd.ie |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048224
- Label: PRIDE project
- Name: Proteolysis pattern of two variants of Chickpea using LC-MS/MS