PXD048223
PXD048223 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Catabolic pathway acquisition by soil pseudomonads readily enables growth with salicyl alcohol but does not affect colonization of Populus roots |
| Description | Horizontal gene transfer (HGT) is a fundamental evolutionary process that plays a key role in bacterial evolution. The likelihood of a successful transfer event is expected to depend on the precise balance of costs and benefits resulting from pathway acquisition. Most experimental analyses of HGT have focused on phenotypes that have large fitness benefits under appropriate selective conditions, such as antibiotic resistance. However, many examples of HGT involve phenotypes that are predicted to provide smaller benefits, such as the ability to catabolize additional carbon sources. We have experimentally reproduced one such HGT event in the laboratory, studying the effects of transferring a pathway for catabolism of the plant-derived aromatic compound salicyl alcohol into soil isolates from the Pseudomonas genus. We find that pathway acquisition enables rapid catabolism of salicyl alcohol with only minor disruptions to existing metabolic and regulatory networks of the new host. However, this new catabolic potential does not confer a measurable fitness advantage during competitive growth in the rhizosphere. We conclude that the phenotype of salicyl alcohol catabolism is readily transferred by HGT but is selectively neutral under environmentally-relevant conditions. We propose that this condition is common and that HGT of many pathways will be self-limiting because the selective benefits are small and negative frequency-dependent. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-11-25 |
| AnnouncementXML | Submission_2024-11-25_06:41:26.279.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Paul Abraham |
| SpeciesList | scientific name: Pseudomonas; NCBI TaxID: 286; scientific name: Populus; common name: poplars; NCBI TaxID: 3689; |
| ModificationList | Oxidation |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-01-03 06:43:32 | ID requested | |
| ⏵ 1 | 2024-11-25 06:41:26 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: bacteria, rhizosphere, salicyl alcohol, proteomics |
Contact List
| Paul Abraham | |
|---|---|
| contact affiliation | ORNL |
| contact email | abrahampe@ornl.gov |
| lab head | |
| Paul Abraham | |
| contact affiliation | ORNL |
| contact email | pav@ornl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093756/ |
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