PXD048212

PXD048212 is an original dataset announced via ProteomeXchange.

Title	CD109 is novel KSHV entry receptor in human endothelial cells and enhances infection during cellular senescence
Description	The aging process is characterized by cellular functional decline and increased susceptibility to infections. Understanding the association between virus infection and aging is crucial for developing effective strategies against viral infections in older individuals. Kaposi's sarcoma-associated herpesvirus (KSHV) infection increases the risk of Kaposi's sarcoma, a vascular cancer prevalent among the elderly without HIV infection. However, the relationship between KSHV pathogenesis and cellular senescence remains unknown. Here, we demonstrate that KSHV infectivity is significantly increased in senescent human endothelial cells due to enhanced binding of virions to cell surface. Proteomic analysis identified caveolin-1 and CD109 that promote KSHV infection and were significantly upregulated in senescent cells. In particular, CD109 is expressed on cell surface and directly interacts with KSHV virions to enhance KSHV infection. Knockout of CD109 abolished while overexpression of CD109 promote KSHV binding to cell surface, and infectivity. These results identify CD109 as a novel KSHV entry receptor that enhances KSHV infection in senescent cells, which might in part explain the higher sensitivity of elder subjects to KSHV infection and Kaposi's sarcoma.
HostingRepository	PRIDE
AnnounceDate	2024-04-17
AnnouncementXML	Submission_2024-04-16_18:26:43.094.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yun Hee Kang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-02 20:36:06	ID requested
⏵ 1	2024-04-16 18:26:43	announced

Dataset with its publication pending

submitter keyword: KSHV,CD109, human endothelial cell, infection, cellular senescence

Myung-Shin Lee
contact affiliation	Department of Microbiology and Immunology, Eulji University School of Medicine,Daejeon, South Korea
contact email	imslee@gmail.com
lab head
Yun Hee Kang
contact affiliation	Eulji University School of Medicine
contact email	yhkang4719@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048212
     1. Label: PRIDE project
     2. Name: CD109 is novel KSHV entry receptor in human endothelial cells and enhances infection during cellular senescence