PXD048205 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MUT-7 exoribonuclease activity and localisation are mediated by an ancient domain |
Description | The MUT-7 family of 3’-5’ exoribonucleases is evolutionarily conserved across the animal kingdom and plays essential roles in small RNA production in the germline. Most MUT-7 homologs carry a C-terminal domain of unknown function named MUT7 C appended to the exoribonuclease domain. Our analysis shows that the MUT7-C is evolutionary ancient, as a minimal version of the domain exists as an individual protein in prokaryotes. Throughout evolution, MUT7-C has acquired different insertions in animals, expanding its functions. C. elegans MUT-7 contains a specific insertion within MUT7-C, which allows binding to MUT 8 and, consequently, MUT-7 recruitment to germ granules. In addition, in C. elegans and human MUT-7, the MUT7 C domain contributes to RNA binding and is thereby crucial for nuclease activity. This RNA-binding function most likely represents the ancestral function of the MUT7 C domain. Overall, this study sheds light on MUT7-C and assigns two functions to this previously uncharacterised domain. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_02:37:16.009.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | WeiQiang Chen |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-02 19:26:20 | ID requested | |
⏵ 1 | 2024-10-17 02:37:16 | announced | |
Publication List
Busetto V, Pshanichnaya L, Lichtenberger R, Hann S, Ketting RF, Falk S, MUT-7 exoribonuclease activity and localization are mediated by an ancient domain. Nucleic Acids Res, 52(15):9076-9091(2024) [pubmed] |
10.1093/nar/gkae610; |
Keyword List
submitter keyword: MUT7-C,MUT-7, RDE-2, MUT-8, Mutator complex |
Contact List
Sebastian Falk |
contact affiliation | Department of Structural and Computational Biology, Center for Molecular Biology, University of Vienna, Campus Vienna Biocenter 5, 1030, Vienna, Austria |
contact email | sebastian.falk@univie.ac.at |
lab head | |
WeiQiang Chen |
contact affiliation | Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH |
contact email | chenweiqiang@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048205
- Label: PRIDE project
- Name: MUT-7 exoribonuclease activity and localisation are mediated by an ancient domain