PXD048205

PXD048205 is an original dataset announced via ProteomeXchange.

Title	MUT-7 exoribonuclease activity and localisation are mediated by an ancient domain
Description	The MUT-7 family of 3’-5’ exoribonucleases is evolutionarily conserved across the animal kingdom and plays essential roles in small RNA production in the germline. Most MUT-7 homologs carry a C-terminal domain of unknown function named MUT7 C appended to the exoribonuclease domain. Our analysis shows that the MUT7-C is evolutionary ancient, as a minimal version of the domain exists as an individual protein in prokaryotes. Throughout evolution, MUT7-C has acquired different insertions in animals, expanding its functions. C. elegans MUT-7 contains a specific insertion within MUT7-C, which allows binding to MUT 8 and, consequently, MUT-7 recruitment to germ granules. In addition, in C. elegans and human MUT-7, the MUT7 C domain contributes to RNA binding and is thereby crucial for nuclease activity. This RNA-binding function most likely represents the ancestral function of the MUT7 C domain. Overall, this study sheds light on MUT7-C and assigns two functions to this previously uncharacterised domain.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:37:16.009.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiQiang Chen
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-02 19:26:20	ID requested
⏵ 1	2024-10-17 02:37:16	announced

Busetto V, Pshanichnaya L, Lichtenberger R, Hann S, Ketting RF, Falk S, MUT-7 exoribonuclease activity and localization are mediated by an ancient domain. Nucleic Acids Res, 52(15):9076-9091(2024) [pubmed]

10.1093/nar/gkae610;

submitter keyword: MUT7-C,MUT-7, RDE-2, MUT-8, Mutator complex

Sebastian Falk
contact affiliation	Department of Structural and Computational Biology, Center for Molecular Biology, University of Vienna, Campus Vienna Biocenter 5, 1030, Vienna, Austria
contact email	sebastian.falk@univie.ac.at
lab head
WeiQiang Chen
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH
contact email	chenweiqiang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD048205

PRIDE project URI

• PRIDE
  1. PXD048205
     1. Label: PRIDE project
     2. Name: MUT-7 exoribonuclease activity and localisation are mediated by an ancient domain