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PXD048203

PXD048203 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of recombinant HupS protein in vitro acetylation extent
DescriptionNucleoid-associated proteins (NAPs) are critical during the process of chromatin compaction in Streptomyces soil bacteria. HupS is one of the two NAPs encoded in the Streptomyces genome. Its unique C-terminal domain, rich in lysine repeats (LR domain), is alike to the H2B histone found in eukaryotic cells or the HupB protein found in Mycobacterium. Project study aim was to investigate if the lysine residues of the HupS LR domain undergo the posttranslational reversible lysine acetylation process. Mass spectrometry approach was employed in order to identify the number of acetyl groups attached to the recombinantly produced and subsequently purified HupS protein after it was subdue to an in vitro acetylation reaction with acetyl phosphate (AcP).
HostingRepositoryPRIDE
AnnounceDate2024-06-03
AnnouncementXMLSubmission_2024-06-03_02:59:23.471.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichał Tracz
SpeciesList scientific name: Streptomyces venezuelae; NCBI TaxID: 54571;
ModificationListacetylated residue
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-02 09:48:12ID requested
12024-06-03 02:59:23announced
Publication List
10.1093/NAR/GKAE418;
Keyword List
submitter keyword: LC-MS, acetylation, NAP protein, HupS, protein modification, Streptomyces, intact protein mass analysis
Contact List
Michal Tracz
contact affiliationLaboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw, Poland
contact emailmichal.tracz2@uwr.edu.pl
lab head
Michał Tracz
contact affiliationLaboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact emailmichal.tracz2@uwr.edu.pl
dataset submitter
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