PXD048203 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of recombinant HupS protein in vitro acetylation extent |
Description | Nucleoid-associated proteins (NAPs) are critical during the process of chromatin compaction in Streptomyces soil bacteria. HupS is one of the two NAPs encoded in the Streptomyces genome. Its unique C-terminal domain, rich in lysine repeats (LR domain), is alike to the H2B histone found in eukaryotic cells or the HupB protein found in Mycobacterium. Project study aim was to investigate if the lysine residues of the HupS LR domain undergo the posttranslational reversible lysine acetylation process. Mass spectrometry approach was employed in order to identify the number of acetyl groups attached to the recombinantly produced and subsequently purified HupS protein after it was subdue to an in vitro acetylation reaction with acetyl phosphate (AcP). |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-03 |
AnnouncementXML | Submission_2024-06-03_02:59:23.471.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michał Tracz |
SpeciesList | scientific name: Streptomyces venezuelae; NCBI TaxID: 54571; |
ModificationList | acetylated residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-02 09:48:12 | ID requested | |
⏵ 1 | 2024-06-03 02:59:23 | announced | |
Publication List
Keyword List
submitter keyword: LC-MS, acetylation, NAP protein, HupS, protein modification, Streptomyces, intact protein mass analysis |
Contact List
Michal Tracz |
contact affiliation | Laboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw, Poland |
contact email | michal.tracz2@uwr.edu.pl |
lab head | |
Michał Tracz |
contact affiliation | Laboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw |
contact email | michal.tracz2@uwr.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048203
- Label: PRIDE project
- Name: Identification of recombinant HupS protein in vitro acetylation extent