PXD048180

PXD048180 is an original dataset announced via ProteomeXchange.

Title	SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23
Description	Here we characterized SRSF1’s interactome using proximity labeling and mass spectrometry. This approach yielded 190 proteins enriched in the SRSF1 samples, independently of the N- or C-terminal location of the biotin-labeling domain. The detected proteins reflect established functions of SRSF1 in pre-mRNA splicing and reveal additional connections to spliceosome proteins, in addition to other recently identified functions. We validated a robust interaction with the spliceosomal RNA helicase DDX23/PRP28 using bimolecular fluorescence complementation and in vitro binding assays. The interaction is mediated by the N-terminal RS-like domain of DDX23 and both RRM1 and the RS domain of SRSF1.
HostingRepository	PRIDE
AnnounceDate	2024-04-30
AnnouncementXML	Submission_2024-04-30_11:10:57.554.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048180
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paolo Cifani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-30 03:37:11	ID requested
⏵ 1	2024-04-30 11:10:58	announced

10.6019/PXD048180;

10.1073/PNAS.2322974121;

submitter keyword: BioID SRSF1 interactome DDX23 affinity proteomics

Paolo Cifani
contact affiliation	Cold Spring Harbor Laboratory - Mass Spectrometry Shared Resource
contact email	cshl.mass.spec@gmail.com
lab head
Paolo Cifani
contact affiliation	Cold Spring Harbor Laboratory
contact email	cshl.mass.spec@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD048180

PRIDE project URI

• PRIDE
  1. PXD048180
     1. Label: PRIDE project
     2. Name: SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23