PXD048177 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A metabolite sensor subunit of the Atg1/ULK complex regulates selective autophagy; Co-IP data |
Description | Cells convert complex metabolic information into stress-adapted autophagy responses. Canonically, multilayered protein kinase networks converge on the conserved Atg1/ULK kinase complex (AKC) to induce non-selective and selective forms of autophagy in response to metabolic changes. Here, we show that, upon phosphate starvation, the metabolite sensor Pho81 interacts with the adaptor subunit Atg11 at the AKC via an Atg11/FIP200 interaction motif to modulate pexophagy by virtue of its conserved phospho-metabolite sensing SPX domain. Notably, core AKC components Atg13 and Atg17 are dispensable for phosphate starvation-induced autophagy revealing significant compositional and functional plasticity of the AKC. Our data indicate that, instead of functioning as a selective autophagy receptor, Pho81 compensates for partially inactive Atg13 by promoting Atg11-phosphorylation by Atg1 critical for pexophagy during phosphate starvation. Our work shows Atg11/FIP200 adaptor subunits not only bind selective autophagy receptors but also modulator subunits that convey metabolic information directly to the AKC for autophagy regulation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-31 |
AnnouncementXML | Submission_2023-12-30_21:31:33.223.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ilian Atanassov |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-29 21:04:29 | ID requested | |
⏵ 1 | 2023-12-30 21:31:33 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: autophagy, IP, LFQ |
Contact List
Martin Graef |
contact affiliation | Department of Molecular Biology and Genetics Cornell University 201 Biotechnology 526 Campus Road Ithaca, NY 14853 |
contact email | mg2255@cornell.edu |
lab head | |
Ilian Atanassov |
contact affiliation | Max Planck Institute for Biology of Aging |
contact email | Ilian.Atanassov@age.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD048177 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048177
- Label: PRIDE project
- Name: A metabolite sensor subunit of the Atg1/ULK complex regulates selective autophagy; Co-IP data