PXD048169

PXD048169 is an original dataset announced via ProteomeXchange.

Title	Simultaneous determination of animal products from ruminants, pig, poultry and fish in feedingstuff by targeted high-resolution LC-MS/MS
Description	The prohibition of feeding processed animal proteins (PAPs) has been relaxed gradually since 2007. The official control method for PAPs in feedingstuff, a combination of light microscopy (LM) followed by PCR is no longer sufficient. Thus, a targeted LC-MS/MS method was developed, which enables a tissue specific distinction between PAPs derived from blood, muscle, skin (gelatine), egg and dairy products of the species ruminants, pig, poultry and fish. Tissue specific proteins were analyzed after reduction, alkylation and tryptic digestion to peptides by data dependent acquisition (DDA) and data independent acquisition (DIA) with high-resolution ESI-QTOF-MS. A targeted method was developed based on untargeted proteomics approaches and selection of specific peptides in accordance with generally applicable criteria. IIdentification of unique peptides for all authorized and non-authorized animal products of the four species was successful. The established method with a total of 45 unique peptides is the first which matches all requirements for feed control due the relaxing of feed ban for PAPs. Proficiency testing of blank and spiked samples revealed excellent results for trueness and selectivity; no false positive or negative results were observed. Furthermore, sensitivity was achieved to a level of 0.1 % (w/w) of the spiked samples that fulfills the EU requirements. Summing up, the developed method seems to be suitable for routine analysis and the performance should be evaluated by further ring trials.
HostingRepository	PanoramaPublic
AnnounceDate	2025-10-02
AnnouncementXML	Submission_2025-10-02_20:05:31.298.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Sara Schlachter
SpeciesList	scientific name: Danio rerio; NCBI TaxID: 7955; scientific name: Bos taurus; NCBI TaxID: 9913; scientific name: Sus scrofa; NCBI TaxID: 9823; scientific name: Gallus gallus; NCBI TaxID: 9031;
ModificationList	Carbamidomethyl
Instrument	Xevo G2-XS QTof; ACQUITY UPLC H-Class Bio

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-29 00:04:06	ID requested
⏵ 1	2025-10-02 20:05:33	announced

Schlachter S, Tadus K, Weiss R, Reiter E, Strnad I, Cichna-Markl M, D'Amico S, Simultaneous Determination of Animal Products from Ruminant, Pig, Poultry, and Fish in Feedingstuff by Targeted High-Resolution LC-MS/MS. J Agric Food Chem, 72(27):15357-15365(2024) [pubmed]

submitter keyword: Processed animal proteins, high-resolution liquid chromatography mass spectrometry, proteomics, parallel reaction monitoring

Elisabeth Reiter
contact affiliation	AGES
contact email	elisabeth.reiter@ages.at
lab head
Sara Schlachter
contact affiliation	AGES
contact email	sara-sofie.schlachter@ages.at
dataset submitter

Panorama Public dataset URI