PXD048167

PXD048167 is an original dataset announced via ProteomeXchange.

Title	Proteome overview of exosome derived from plasma of cows infected with Mycobacterium bovis
Description	Bovine tuberculosis (bTB), primarily caused by Mycobacterium bovis (M. bovis), is a globally zoonotic disease with significant economic impacts. Plasma exosomes have been extensively used for investigating disease processes and exploring biomarkers. While mass spectrometry (MS)-based proteomic analysis of plasma exosomes has been employed for human tuberculosis (TB) studies, it has not yet been applied to bTB. Therefore, a comprehensive proteomic overview of plasma exosomes from M. bovis-infected cows is essential. In this study, we present an extensive proteomic analysis of plasma exosomes from 89 M. bovis-infected cows across three farms, using data-dependent acquisition (DDA) mode. Our analysis encompasses 239,894 spectra, 6,011 peptides and 835 protein groups. The proteomic overview reveals both consistencies and differences among individual cows, supplements 595 proteins to the bovine exosome library, and enriches tuberculosis and related pathways. Additionally, six pathways were validated as immune response pathways, and three proteins (CATHL1, H1-1, and LCN2) were identified as potential indicators of bTB. This study is the first to investigate the exosome proteome of plasma from cows infected with M. bovis, providing a valuable dataset for exploring candidate bTB markers and understanding the mechanisms of host defense against M. bovis.
HostingRepository	iProX
AnnounceDate	2024-06-17
AnnouncementXML	Submission_2024-07-02_19:20:20.815.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yao Zhang
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-28 20:34:19	ID requested
⏵ 1	2024-07-02 19:20:21	announced

Dataset with its publication pending

submitter keyword: bovine tuberculosis, plasma exosome, proteome, mass spectrometry

Ping Xu
contact affiliation	Beijing Proteome Research Center
contact email	xuping_bprc@126.com
lab head
Yao Zhang
contact affiliation	Beijing Proteome Research Center
contact email	zhangyaowsw@163.com
dataset submitter

iProX dataset URI