Update information. To elucidate the molecular mechanism and search for EIF3H substrate(s) of EIF3H-mediated CRC progression, we performed immune-affinity purification and resolved the binding partners utilizing mass spectrometry. HCT116 cells transfected with empty vector or Flag-tagged EIF3H and then immunoprecipitated using anti-Flag M2 beads.The purified EIF3H protein complex was subjected to mass spectrometry analysis.