PXD048149

PXD048149 is an original dataset announced via ProteomeXchange.

Title	Deciphering the role of MFGE8 in lactation biology using CRISPR-CAS9 based gene editing in Buffalo mammary epithelial cells
Description	Milk fat globule EGF factor 8 (MFGE8) is a glycoprotein which plays a crucial role in mammary gland remodeling. Our group previously identified MFGE8 as a marker associated with high milk yielding cows. Here, we generated MFGE8 knock-out buffalo mammary epithelial cells (BuMEC) via CRISPR-cas9 technology to decipher its role in lactation. gRNA3 reduced MFGE8 expression with good efficiency which was confirmed at transcriptomic and proteomic level and the stable knock-out cells obtained were named mfge8-/-/gRNA3. The amplicon sequencing of the edited region using next generation sequencing (NGS) showed that 54% of total reads showed indels, 3-4 bp upstream to PAM site in 2nd exon. A total 4282 proteins were identified when proteome level changes were examined and 178 were found to be differentially expressed above and below a threshold of ≥1.5 and ≤0.6. Major DEPs were found to be associated with regulation of hydrolase activity, endopeptidase activity and cytoskeletal organization and some DEPs including FABP3, FABP4, FABP5, KNG1, MT2A, CD82 and SERPINH1 belonged to genes associated with milk synthesis. To the best of our knowledge, this is the first study which provides a comprehensive proteome profile of MFGE8 knockout BuMEC and explores downstream effects of disruption of MFGE8 gene.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:14:36.099.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PREETI VATS
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-27 11:12:26	ID requested
⏵ 1	2025-08-24 16:14:36	announced

10.1038/s41598-025-00155-7;

Vats P, Kumar R, Kumar R, Kaushik JK, Mohanty AK, Kumar S, Deciphering the role of MFGE8 in lactation using CRISPR-CAS9 based gene editing in Buffalo mammary epithelial cells. Sci Rep, 15(1):30194(2025) [pubmed]

submitter keyword: CRISPR-CAS9, lactation biology,MFGE8

Dr. Sudarshan Kumar
contact affiliation	Senior Scientist, Proteomics and cell biology Lab, Animal biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
contact email	kumarsudershan@gmail.com
lab head
PREETI VATS
contact affiliation	RESEARCH ASSOCIATE
contact email	bhardwajp10@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD048149

PRIDE project URI

• PRIDE
  1. PXD048149
     1. Label: PRIDE project
     2. Name: Deciphering the role of MFGE8 in lactation biology using CRISPR-CAS9 based gene editing in Buffalo mammary epithelial cells