PXD048143

PXD048143 is an original dataset announced via ProteomeXchange.

Title	Adaptive laboratory evolution of Clostridium autoethanogenum to metabolize CO2 and H2 enhances growth rates in chemostat and unravels proteome and metabolome alterations
Description	Gas fermentation of CO₂ and H₂ is an attractive means to sustainably produce fuels and chemicals. Clostridium autoethanogenum is a model organism for industrial CO-to-ethanol and presents an opportunity for CO₂-to-ethanol processes. As we have previously characterized its CO₂/H₂ chemostat growth, here we use adaptive laboratory evolution (ALE) with the aim of improving growth with CO₂/H₂. Seven ALE lineages were generated, all with improved specific growth rates. Developed with 2% CO supplementation of CO₂/H₂, Evolved lineage D has the highest ethanol/acetate of ALE lineages when fermenting CO₂/H₂. Chemostat comparison against the parental strain shows no change in acetate or ethanol production, while Evolved D could achieve a higher maximum dilution rate. Complete multi-omics analyses at steady-state revealed that although Evolved D has widespread proteome changes, intracellular metabolites prevent phenotype shifts. Yet, we observe numerous insights to CO₂/H₂ metabolism via these multi-omics results and link these to mutations, suggesting novel targets for metabolic engineering.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:38:43.592.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Heffernan
SpeciesList	scientific name: Clostridium autoethanogenum; NCBI TaxID: 84023;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-27 10:40:49	ID requested
1	2024-05-09 15:58:48	announced
⏵ 2	2024-10-22 06:38:43	announced	2024-10-22: Updated project metadata.

10.1111/1751-7915.14452;

submitter keyword: ALE, Clostridium autoethanogenum, acetogen,Gas fermentation

Dr Esteban
contact affiliation	Australian Institute for Bioengineering and Nanotechnology, The University of Queensland
contact email	e.marcellin@uq.edu.au
lab head
James Heffernan
contact affiliation	Australian Institute for Bioengineering and Nanotechnology, The University of Queensland
contact email	j.heffernan@uq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048143
     1. Label: PRIDE project
     2. Name: Adaptive laboratory evolution of Clostridium autoethanogenum to metabolize CO2 and H2 enhances growth rates in chemostat and unravels proteome and metabolome alterations