PXD048128 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS after Immunoprecipitation with Mbd5 in zebrafish at 4dpf |
Description | To address how Mbd5 binding to m5C RNAs mediates transcriptional activation of its target genes. Using the Tg[zhsp70l:FLAGmbd5iso2-E2AGFP] zebrafish, we performed heat shock at multiple developmental stages and carried out co-immunoprecipitation (co-IP) at 4 dpf using the FLAG antibody, followed by mass spectrometry |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:45:36.183.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jianhua Guo |
SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-25 20:52:58 | ID requested | |
1 | 2024-06-16 01:13:56 | announced | |
⏵ 2 | 2024-10-22 06:45:38 | announced | 2024-10-22: Updated project metadata. |
Publication List
Guo J, Zou Z, Dou X, Zhao X, Wang Y, Wei L, Pi Y, Wang Y, He C, Guo S, Zebrafish Mbd5 binds to RNA m5C and regulates histone deubiquitylation and gene expression in development metabolism and behavior. Nucleic Acids Res, 52(8):4257-4275(2024) [pubmed] |
10.1093/nar/gkae093; |
Keyword List
submitter keyword: zebrafish Mbd5 Co-IP |
Contact List
Su Guo |
contact affiliation | Department of Bioengineering and Therapeutic Sciences, Programs in Human Genetics and Biological Sciences, University of California, San Francisco, CA 94143, USA |
contact email | Su.Guo@ucsf.edu |
lab head | |
Jianhua Guo |
contact affiliation | College of Life Sciences, Fudan university |
contact email | jhguo289863667@outlook.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048128
- Label: PRIDE project
- Name: LC-MS/MS after Immunoprecipitation with Mbd5 in zebrafish at 4dpf