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PXD048128

PXD048128 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS after Immunoprecipitation with Mbd5 in zebrafish at 4dpf
DescriptionTo address how Mbd5 binding to m5C RNAs mediates transcriptional activation of its target genes. Using the Tg[zhsp70l:FLAGmbd5iso2-E2AGFP] zebrafish, we performed heat shock at multiple developmental stages and carried out co-immunoprecipitation (co-IP) at 4 dpf using the FLAG antibody, followed by mass spectrometry
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:45:36.183.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJianhua Guo
SpeciesList scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-25 20:52:58ID requested
12024-06-16 01:13:56announced
22024-10-22 06:45:38announced2024-10-22: Updated project metadata.
Publication List
Guo J, Zou Z, Dou X, Zhao X, Wang Y, Wei L, Pi Y, Wang Y, He C, Guo S, Zebrafish Mbd5 binds to RNA m5C and regulates histone deubiquitylation and gene expression in development metabolism and behavior. Nucleic Acids Res, 52(8):4257-4275(2024) [pubmed]
10.1093/nar/gkae093;
Keyword List
submitter keyword: zebrafish Mbd5 Co-IP
Contact List
Su Guo
contact affiliationDepartment of Bioengineering and Therapeutic Sciences, Programs in Human Genetics and Biological Sciences, University of California, San Francisco, CA 94143, USA
contact emailSu.Guo@ucsf.edu
lab head
Jianhua Guo
contact affiliationCollege of Life Sciences, Fudan university
contact emailjhguo289863667@outlook.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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