PXD048123

PXD048123 is an original dataset announced via ProteomeXchange.

Title	Effects of BfmR Overexpression and Signal Induction Optimization on Pseudomonas Siderophore Synthesis for Iron Removal
Description	Pseudomonas and other environmental microorganisms have been proven capable of synthesizing siderophores, which are instrumental in the removal of iron and other metal ions from a variety of waste materials. We have elucidated the molecular mechanisms by which the BfmRS two-component system (TCS) mediates environmental stress signals to regulate siderophore production in Pseudomonas aeruginosa. In this study, we further confirm the pivotal role of the BfmRS system in bacterial iron metabolism, demonstrating its regulatory influence on key genes involved in siderophore synthesis. Moreover, overexpression of BfmR led to a marked increase in mRNA levels of siderophore-related genes, with a 2.4- to 6.7-fold elevation, which in turn significantly enhanced siderophore production and consequently improved iron utilization efficiency when compared to the wild type (WT). The heightened efficiency of the genetically modified strain to extract iron from coal fly ash (CFA) suggests the feasibility of engineered bacterial system in bioremediation. This work not only validate the intricate TCSs involved in siderophore regulation within P. aeruginosa, but also provides a compelling strategy in heavy metal recovery and hazardous waste detoxification.
HostingRepository	iProX
AnnounceDate	2023-12-25
AnnouncementXML	Submission_2023-12-25_19:26:16.843.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ze Li
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	No PTMs are included in the dataset
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-25 19:20:41	ID requested
⏵ 1	2023-12-25 19:26:17	announced

Dataset with its publication pending

submitter keyword: Pseudomonas aeruginosa, two component system,proteomic analysis,siderophore regulation,iron removal

Rui Bao
contact affiliation	Center of Infectious Diseases, Division of Infectious Diseases in State Key Laboratory of Biotherapy, West China Hospital, Sichuan University
contact email	baorui@scu.edu.cn
lab head
Ze Li
contact affiliation	State Key Laboratory of Biotherapy, , Sichuan University, Chengdu 610041, ChinaSichuan University
contact email	1477854366@qq.com
dataset submitter

iProX dataset URI