PXD048082

PXD048082 is an original dataset announced via ProteomeXchange.

Title	N-terminal acetylation of yeast NAT-A mutants
Description	Protein-protein interactions are fundamental to all cellular activities, with the ribosome increasingly recognized as a central platform steering the dynamics of nascent-chain interactions. In our focus on conserved N-terminal acetyltransferases (NATs), we identified distinct co-translational assembly pathways. This distinction persisted even among highly homologous subunits, with some playing contrasting roles. Notably, we found that only a few residues act as “hotspots”, initiating co-translational assembly interactions as they emerge from the ribosome exit tunnel. The significance of these hotspots was further validated in vivo through N-terminomics studies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:46:21.342.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oded Kleifeld
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-22 07:33:31	ID requested
1	2024-06-22 08:29:00	announced
⏵ 2	2024-10-22 06:46:22	announced	2024-10-22: Updated project metadata.

10.1038/s41467-024-46881-w;

Venezian J, Bar-Yosef H, Ben-Arie Zilberman H, Cohen N, Kleifeld O, Fernandez-Recio J, Glaser F, Shiber A, Diverging co-translational protein complex assembly pathways are governed by interface energy distribution. Nat Commun, 15(1):2638(2024) [pubmed]

submitter keyword: N-terminal acetylation, NAA15, NAA10,Co-translational assembly, Terminomics

Ayala Shiber
contact affiliation	Faculty of Biology, Technion – Israel Institute of Technology Haifa 32000, Israel
contact email	ayalashiber@technion.ac.il
lab head
Oded Kleifeld
contact affiliation	Technion
contact email	okleifeld@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD048082

PRIDE project URI

• PRIDE
  1. PXD048082
     1. Label: PRIDE project
     2. Name: N-terminal acetylation of yeast NAT-A mutants