PXD048077

PXD048077 is an original dataset announced via ProteomeXchange.

Title	NDUFAF3 is involved in the assembly of the Q/P module of respiratory complex I in the green microalga Chlamydomonas reinhardtii
Description	The mitochondrial NADH:ubiquinone oxidoreductase, or complex I, is composed of a hydrophobic arm comprising the P module and a hydrophilic arm comprising the N and Q modules. The assembly of complex I is well characterized in humans and catalyzed by a series of assembly factors that join the Q, P, and N modules sequentially. The complex I of protists and plants contains additional ancestral features, like the ferredoxin bridge that connects the matrix and the membrane arms and the  carbonic anhydrase domain, which raises the question of their assembly. In this work, a strain where the assembly factor NDUFAF3 has been tagged with a 3xFLAG at the C-terminal extremity has been investigated in the green microalga Chlamydomonas reinhardtii. Like its human homolog, NDUFAF3 interacts strongly with the classical subunits of the Q and P modules, but also with the  carbonic anhydrase domain and C1-FDX, one of the subunits of the ferredoxin bridge. The predicted structural positioning of NDUFAF3 within the Q module suggests a role in the formation of the ferredoxin bridge. In contrast, subunits of the N module are only loosely associated with NDUFAF3. We further demonstrate that the N module is attached at a later stage of assembly, suggesting that Chlamydomonas complex I assembles in a human-like pathway. This contrasts with the situation in Angiosperms where the N and the Q modules are attached together before anchoring to the P module. Altogether, these results highlight a conserved and ancestral role of NDUFAF3 in complex I manufacture.
HostingRepository	PRIDE
AnnounceDate	2025-07-15
AnnouncementXML	Submission_2025-07-15_00:59:49.381.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD048077
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Johana Chicher
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-22 04:33:10	ID requested
⏵ 1	2025-07-15 00:59:49	announced

10.6019/PXD048077;

10.1111/PPL.70406;

submitter keyword: assembly factor, NDUFAF3, ferredoxin bridge, complex I, mitochondrial respiratory chain,Chlamydomonas

Thalia Salinas-Giegé
contact affiliation	Institut de biologie moleculaire des plantes, CNRS, Universite de Strasbourg, Strasbourg, France
contact email	thalia.salinas@ibmp-cnrs.unistra.fr
lab head
Johana Chicher
contact affiliation	Université de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche Scientifique
contact email	j.chicher@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD048077

PRIDE project URI

• PRIDE
  1. PXD048077
     1. Label: PRIDE project
     2. Name: NDUFAF3 is involved in the assembly of the Q/P module of respiratory complex I in the green microalga Chlamydomonas reinhardtii