PXD048064

PXD048064 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Global phosphoproteomics for CRISPR/Cas9 mediated PTPN22 Ser325 mutagenesis on Jurkat cells
Description	LQF of 3x FLAG PTPN22 WT Jurkat cells compared to S325A and S325E KI mutant Jurkat cells. WT or KI lines were stimulated with or without TCR engagement for 5 min.
HostingRepository	MassIVE
AnnounceDate	2024-12-03
AnnouncementXML	Submission_2024-12-03_15:48:10.722.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Leigh-Ana Rossitto
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Phospho; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-21 18:41:12	ID requested
⏵ 1	2024-12-03 15:48:11	announced

Publication List

Zhuang C, Yang S, Gonzalez CG, Ainsworth RI, Li S, Kobayashi MT, Wierzbicki I, Rossitto LM, Wen Y, Peti W, Stanford SM, Gonzalez DJ, Murali R, Santelli E, Bottini N, A novel gain-of-function phosphorylation site modulates PTPN22 inhibition of TCR signaling. J Biol Chem, 300(6):107393(2024) [pubmed]

Zhuang C, Yang S, Gonzalez CG, Ainsworth RI, Li S, Kobayashi MT, Wierzbicki I, Rossitto LM, Wen Y, Peti W, Stanford SM, Gonzalez DJ, Murali R, Santelli E, Bottini N, A novel gain-of-function phosphorylation site modulates PTPN22 inhibition of TCR signaling. J Biol Chem, 300(6):107393(2024) [pubmed]

Keyword List

submitter keyword: PTPN22, Protein tyrosine kinase, Protein tyrosine phosphatase, T cell signaling, Phosphoproteomics, Jurkat cells

submitter keyword: PTPN22, Protein tyrosine kinase, Protein tyrosine phosphatase, T cell signaling, Phosphoproteomics, Jurkat cells

Contact List

Nunzio Bottini
contact affiliation	ACTRI
contact email	nbottini@ucsd.edu
lab head
David J Gonzalez
contact affiliation	UCSD
contact email	djgonzalez@health.ucsd.edu
lab head
Leigh-Ana Rossitto
contact affiliation	UCSD
contact email	lrossitt@health.ucsd.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093718/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093718/