PXD048052 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A zwitterionic detergent and catalyst-based single-cell proteomics using a loss-free microhole-collection disc |
Description | Recent advances in single-cell proteomics have solved many bottlenecks, such as throughput, sample recovery, and scalability via nano-scale sample handling. In this study, we aimed for a sensitive mass spectrometry analysis capable of handling single cells with a conventional mass spectrometry workflow without additional equipment. We achieved seamless cell lysis and TMT-labeling in a micro-HOLe Disc (microHOLD single-cell proteomics) by developing a mass-compatible single solution based on a zwitterionic detergent and a catalyst for single-cell lysis and tandem mass tag-labeling without a heat incubation step. This method was developed to avoid peptide loss by surface absorption and buffer or tube changes by collecting tandem mass tag-labeled peptide through microholes placed in the liquid chromatography injection vials in a single solution. We successfully applied the microHOLD single-cell proteomics method for the analysis of proteome reprogramming in hormone-sensitive prostate cells to develop castration-resistant prostate cancer cells. This novel single-cell proteomic method is not limited by cutting-edge nano-volume handling equipment and achieves high-throughput and ultrasensitive proteomic analysis of limited samples, such as single cells. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-28 |
AnnouncementXML | Submission_2025-05-28_08:31:33.883.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jaemyung Jang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-21 10:12:18 | ID requested | |
⏵ 1 | 2025-05-28 08:31:34 | announced | |
Publication List
10.1021/acs.analchem.4c00158; |
Yeo S, Jang J, Jung HJ, Lee H, Lee S, Choe Y, A Zwitterionic Detergent and Catalyst-Based Single-Cell Proteomics Using a Loss-Free Microhole-Collection Disc. Anal Chem, 96(29):11690-11698(2024) [pubmed] |
Keyword List
submitter keyword: zwitterionic detergent, Tandem Mass Tags, quadrol,single-cell proteomics |
Contact List
Youngshik Choe |
contact affiliation | Korea Brain Research Institute, Daegu, 41068, Korea |
contact email | dallarae@kbri.re.kr |
lab head | |
Jaemyung Jang |
contact affiliation | Korea Brain Research Institute |
contact email | piloter2@kbri.re.kr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD048052 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048052
- Label: PRIDE project
- Name: A zwitterionic detergent and catalyst-based single-cell proteomics using a loss-free microhole-collection disc