PXD048039

PXD048039 is an original dataset announced via ProteomeXchange.

Title	The Role of Extracellular Vesicle Fusion with Target Cells in Triggering Systemic Inflammation
Description	Extracellular vesicles (EVs) play a crucial role in intercellular communication by transferring bioactive molecules from donor to recipient cells. As a result, EV fusion leads to the modulation of cellular functions and has an impact on both physiological and pathological processes in the recipient cell. This study explores the impact of EV fusion on cellular responses to inflammatory signaling. Our findings reveal that fusion renders non-responsive cells susceptible to inflammatory signaling, as evidenced by increased NF-κB activation and the release of inflammatory mediators. Syntaxin-binding protein 1 is essential for the merge and activation of intracellular signaling. Subsequent analysis revealed that EVs transfer their functionally active receptors to target cells, making them prone to an otherwise unresponsive state. EVs in complex with their agonist, require no further stimulation of the target cells to trigger mobilization of NF-B. While receptor antagonists were unable to inhibit NF-B activation, blocking of the fusion between EVs and their target cells with heparin mitigated inflammation in mice challenged with EVs.
HostingRepository	PRIDE
AnnounceDate	2024-02-21
AnnouncementXML	Submission_2024-02-21_01:20:47.026.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Core Facility
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-21 05:08:20	ID requested
⏵ 1	2024-02-21 01:20:47	announced

10.1038/s41467-024-45125-1;

Papareddy P, Tapken I, Kroh K, Varma Bhongir RK, Rahman M, Baumgarten M, Cim EI, Gy, ö, rffy L, Smeds E, Neumann A, Veerla S, Olinder J, Thorlacus H, Ryden C, Bartakova E, Holub M, Herwald H, The role of extracellular vesicle fusion with target cells in triggering systemic inflammation. Nat Commun, 15(1):1150(2024) [pubmed]

submitter keyword: exosomes, TNFR1, receptors,Extracellular vesicles, fusion, IL1R1 and phosphorylation, STXBP1

Praveen Papareddy
contact affiliation	Division of Infection Medicine, Department of Clinical Sciences, Lund, Lund University, Biomedical Center, Tornavägen 10, SE-22184 Lund, Sweden
contact email	praveen.papareddy@med.lu.se
lab head
Proteomics Core Facility
contact affiliation	SAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg
contact email	gupcf@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD048039

PRIDE project URI

• PRIDE
  1. PXD048039
     1. Label: PRIDE project
     2. Name: The Role of Extracellular Vesicle Fusion with Target Cells in Triggering Systemic Inflammation