PXD048037

PXD048037 is an original dataset announced via ProteomeXchange.

Title	Multiomics analysis of Morus alba cell cultures elicited with methyl-beta-cyclodextrin and methyl jasmonate to investigate stilbene metabolism
Description	In this study, M. alba cell suspension culture established after young stems were treated with elicitors methyl-beta-cyclodextrin combined with methyl jasmonate at 50mM and 0.1mM concentration, respectively. Elicited and control cell cultures were sampled at 0, 6, 12, 24, 48 and 72 hours and used for stilbene analysis in both the extracellular and intracellular medium, and for RNA and protein extraction. RNAseq and label-free proteomic analysis was then performed. All time point series were used for transcriptomic and 24, 48 and 72 hours time points the proteomic analysis. Transcriptomic data were used to reannotate the genome draft published by (Jiao et al. (2020), Molecular Plant, 13(7), 1001-1012) and to update the M. alba transcript and peptide database. MS-based protein identification greatly benefit from the reannotated database. A multiomics analysis was performed to search for candidates of stilbene biosynthesis in M. alba.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_17:14:04.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roque Bru-Martinez
SpeciesList	scientific name: Morus alba; NCBI TaxID: NEWT:3498;
ModificationList	iodoacetamide derivatized residue
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-21 03:07:41	ID requested
⏵ 1	2026-09-06 17:14:05	announced

Santiago A, Pizzio GA, Romero P, Mart, í, nez-M, á, rquez A, Mart, í, nez-Esteso MJ, Echeverria J, Selles-Marchart S, Alvarez-Urdiola R, Zhang C, Navarro-Pay, á D, Mic, ó E, Samper-Herrero A, Morante-Carriel J, Cigliano RA, Manzano D, Bru-Mart, í, nez R, Matus JT, Integrated multi-omics analyses reveal that p-coumaroyl-CoA 2'-hydroxylases act upstream of stilbene synthases to mediate oxyresveratrol biosynthesis in mulberry (Morus alba). Plant Commun, 7(7):101751(2026) [pubmed]

10.1016/j.xplc.2026.101751;

submitter keyword: UHPLC-MS/MS, cell culture, elicitation, temporal series,Morus alba, label-free quantitation

Roque Bru-Martinez
contact affiliation	Department of Biochemistry and Molecular Biology and Agricultural Chemistry and Soil science University of Alicante SPAIN
contact email	roque.bru@ua.es
lab head
Roque Bru-Martinez
contact affiliation	Department of Biochemistry and Molecular Biology and Soil Science and Agricultural Chemistry Faculty of Science. University of Alicante.
contact email	roque.bru@ua.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD048037
     1. Label: PRIDE project
     2. Name: Multiomics analysis of Morus alba cell cultures elicited with methyl-beta-cyclodextrin and methyl jasmonate to investigate stilbene metabolism