PXD048018

PXD048018 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial ATP generation is more proteome efficient than glycolysis (S. cerevisiae)
Description	Metabolic efficiency profoundly influences organismal fitness. Heterotrophs, from yeast to mammals, derive usable energy primarily through glycolysis and respiration. While respiration is more energy-efficient, some cells favor glycolysis even when oxygen is available (aerobic glycolysis, Warburg effect). A leading explanation is that glycolysis is more efficient in terms of ATP production per unit mass of protein (i.e. faster). Through quantitative flux analysis and proteomics, we find however that mitochondrial respiration is actually more proteome-efficient than aerobic glycolysis. This is shown across yeasts, T cells, cancer cells, and tissues and tumors in vivo. Instead of aerobic glycolysis being valuable for fast ATP production, it correlates with high glycolytic protein expression, which is valuable for hypoxic growth. Aerobic glycolytic yeasts do not excel at aerobic growth, but outgrow respiratory cells in oxygen limitation. Thus, aerobic glycolysis emerges from cells maintaining a proteome conducive to both aerobic and hypoxic growth.
HostingRepository	PRIDE
AnnounceDate	2024-04-25
AnnouncementXML	Submission_2024-04-25_13:54:23.969.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edward Cruz
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	TMT6plex reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-20 20:14:41	ID requested
⏵ 1	2024-04-25 13:54:25	announced

10.1038/s41589-024-01571-y;

Shen Y, Dinh HV, Cruz ER, Chen Z, Bartman CR, Xiao T, Call CM, Ryseck RP, Pratas J, Weilandt D, Baron H, Subramanian A, Fatma Z, Wu ZY, Dwaraknath S, Hendry JI, Tran VG, Yang L, Yoshikuni Y, Zhao H, Maranas CD, W, ü, hr M, Rabinowitz JD, Mitochondrial ATP generation is more proteome efficient than glycolysis. Nat Chem Biol, ():(2024) [pubmed]

submitter keyword: Orbitrap Fusion Lumos, absolute proteomics, Saccharomyces cerevisiae

Martin Wuhr
contact affiliation	Molecular Biology and the Lewis-Sigler Institute for Integrative Genomics, Princeton University, USA
contact email	wuhr@princeton.edu
lab head
Edward Cruz
contact affiliation	Princeton University
contact email	ercruz@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD048018

PRIDE project URI

• PRIDE
  1. PXD048018
     1. Label: PRIDE project
     2. Name: Mitochondrial ATP generation is more proteome efficient than glycolysis (S. cerevisiae)