PXD048012 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mitochondrial ATP generation is more proteome efficient than glycolysis (I. orientalis) |
Description | Metabolic efficiency profoundly influences organismal fitness. Heterotrophs, from yeast to mammals, derive usable energy primarily through glycolysis and respiration. While respiration is more energy-efficient, some cells favor glycolysis even when oxygen is available (aerobic glycolysis, Warburg effect). A leading explanation is that glycolysis is more efficient in terms of ATP production per unit mass of protein (i.e. faster). Through quantitative flux analysis and proteomics, we find however that mitochondrial respiration is actually more proteome-efficient than aerobic glycolysis. This is shown across yeasts, T cells, cancer cells, and tissues and tumors in vivo. Instead of aerobic glycolysis being valuable for fast ATP production, it correlates with high glycolytic protein expression, which is valuable for hypoxic growth. Aerobic glycolytic yeasts do not excel at aerobic growth, but outgrow respiratory cells in oxygen limitation. Thus, aerobic glycolysis emerges from cells maintaining a proteome conducive to both aerobic and hypoxic growth. |
HostingRepository | PRIDE |
AnnounceDate | 2024-04-25 |
AnnouncementXML | Submission_2024-04-25_13:45:48.685.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Edward Cruz |
SpeciesList | scientific name: Pichia kudriavzevii; NCBI TaxID: 4909; |
ModificationList | TMT6plex reporter+balance reagent acylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-20 11:07:16 | ID requested | |
⏵ 1 | 2024-04-25 13:45:49 | announced | |
Publication List
10.1038/s41589-024-01571-y; |
Shen Y, Dinh HV, Cruz ER, Chen Z, Bartman CR, Xiao T, Call CM, Ryseck RP, Pratas J, Weilandt D, Baron H, Subramanian A, Fatma Z, Wu ZY, Dwaraknath S, Hendry JI, Tran VG, Yang L, Yoshikuni Y, Zhao H, Maranas CD, W, ΓΌ, hr M, Rabinowitz JD, Mitochondrial ATP generation is more proteome efficient than glycolysis. Nat Chem Biol, ():(2024) [pubmed] |
Keyword List
submitter keyword: Orbitrap Fusion Lumos, Issatchenkia orientalis, absolute proteomics |
Contact List
Martin Wuhr |
contact affiliation | Molecular Biology and the Lewis-Sigler Institute for Integrative Genomics, Princeton University, USA |
contact email | wuhr@princeton.edu |
lab head | |
Edward Cruz |
contact affiliation | Princeton University |
contact email | ercruz@princeton.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048012
- Label: PRIDE project
- Name: Mitochondrial ATP generation is more proteome efficient than glycolysis (I. orientalis)