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PXD048008

PXD048008 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAssembly and release of a Tc toxin
DescriptionTripartite Tc toxins are virulence factors of bacterial pathogens. Although their structure and mechanism of action are well understood, it remains elusive where this large macromolecular complex is assembled and how it is released. Here we show by an integrative multiscale imaging approach that Yersinia entomophaga Tc (YenTc) toxin components are expressed only in a subpopulation of cells that are “primed” with several other potential virulence factors, including filaments of the protease M66/StcE. A phage-like lysis cassette (LC) is required for YenTc release; however, before resulting in complete cell lysis, the LC generates intermediate “ghost” cells, which may serve as assembly compartments and become densely packed with assembled YenTc holotoxins. We hypothesize that this stepwise mechanism evolved to minimize the number of cells that need to be sacrificed. The occurrence of similar lysis cassettes in diverse organisms indicates a conserved mechanism for Tc toxin release that may apply to other extracellular macromolecular machines.
HostingRepositoryPRIDE
AnnounceDate2024-02-20
AnnouncementXMLSubmission_2024-02-20_02:48:10.042.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMiki Feldmüller
SpeciesList scientific name: Yersinia entomophaga; NCBI TaxID: 935293;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-20 08:18:09ID requested
12024-02-20 02:48:11announced
Publication List
10.1038/s41564-024-01611-2;
Feldm, ü, ller M, Ericson CF, Afanasyev P, Lien YW, Weiss GL, Wollweber F, Schoof M, Hurst M, Pilhofer M, Stepwise assembly and release of Tc toxins from Yersinia entomophaga. Nat Microbiol, 9(2):405-420(2024) [pubmed]
Keyword List
submitter keyword: toxins
Contact List
Prof. Martin Pilhofer
contact affiliationETH Zurich Institute of Molecular Biology & Biophysics
contact emailpilhofer@biol.ethz.ch
lab head
Miki Feldmüller
contact affiliationETH Zürich
contact emailfmiki@mol.biol.ethz.ch
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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