PXD048008

PXD048008 is an original dataset announced via ProteomeXchange.

Title	Assembly and release of a Tc toxin
Description	Tripartite Tc toxins are virulence factors of bacterial pathogens. Although their structure and mechanism of action are well understood, it remains elusive where this large macromolecular complex is assembled and how it is released. Here we show by an integrative multiscale imaging approach that Yersinia entomophaga Tc (YenTc) toxin components are expressed only in a subpopulation of cells that are “primed” with several other potential virulence factors, including filaments of the protease M66/StcE. A phage-like lysis cassette (LC) is required for YenTc release; however, before resulting in complete cell lysis, the LC generates intermediate “ghost” cells, which may serve as assembly compartments and become densely packed with assembled YenTc holotoxins. We hypothesize that this stepwise mechanism evolved to minimize the number of cells that need to be sacrificed. The occurrence of similar lysis cassettes in diverse organisms indicates a conserved mechanism for Tc toxin release that may apply to other extracellular macromolecular machines.
HostingRepository	PRIDE
AnnounceDate	2024-02-20
AnnouncementXML	Submission_2024-02-20_02:48:10.042.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miki Feldmüller
SpeciesList	scientific name: Yersinia entomophaga; NCBI TaxID: 935293;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-20 08:18:09	ID requested
⏵ 1	2024-02-20 02:48:11	announced

10.1038/s41564-024-01611-2;

Feldm, ü, ller M, Ericson CF, Afanasyev P, Lien YW, Weiss GL, Wollweber F, Schoof M, Hurst M, Pilhofer M, Stepwise assembly and release of Tc toxins from Yersinia entomophaga. Nat Microbiol, 9(2):405-420(2024) [pubmed]

submitter keyword: toxins

Prof. Martin Pilhofer
contact affiliation	ETH Zurich Institute of Molecular Biology & Biophysics
contact email	pilhofer@biol.ethz.ch
lab head
Miki Feldmüller
contact affiliation	ETH Zürich
contact email	fmiki@mol.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD048008

PRIDE project URI

• PRIDE
  1. PXD048008
     1. Label: PRIDE project
     2. Name: Assembly and release of a Tc toxin