PXD047946

PXD047946 is an original dataset announced via ProteomeXchange.

Title	Identification of MIMAS, a multifunctional mega-assembly integrating metabolic and respiratory biogenesis factors of mitochondria
Description	The mitochondrial inner membrane plays central roles in bioenergetics and metabolism and contains well established membrane protein complexes. Here we report the identification of a megacomplex of the inner membrane, termed mitochondrial multifunctional assembly (MIMAS). Its large size of 3 MDa explains why MIMAS has escaped detection in the analysis of mitochondria so far. MIMAS combines proteins of diverse functions from respiratory assembly to metabolite transport, dehydrogenases and lipid biosynthesis, but not the large established supercomplexes of the respiratory chain, ATP synthase or prohibitin scaffold. MIMAS integrity depends on the non-bilayer phospholipid phosphatidylethanolamine in contrast to respiratory supercomplexes whose stability depends on cardiolipin. Our findings suggest that MIMAS forms a protein-lipid mega-assembly in the mitochondrial inner membrane that integrates respiratory biogenesis and metabolic processes in a multifunctional platform.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:31:28.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Hardt
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-19 06:07:52	ID requested
1	2024-02-23 03:17:55	announced
⏵ 2	2024-10-22 06:31:29	announced	2024-10-22: Updated project metadata.

10.1016/J.CELREP.2024.113772;

submitter keyword: innern membrane, yeast,mitochondria, MIMAS, blue native, megacomplex

Thomas Becker
contact affiliation	Institute for Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, 53115 Bonn, Germany
contact email	thbecker@uni-bonn.de
lab head
Robert Hardt
contact affiliation	Institute for Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, 53115 Bonn, Germany
contact email	rhardt@uni-bonn.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047946
     1. Label: PRIDE project
     2. Name: Identification of MIMAS, a multifunctional mega-assembly integrating metabolic and respiratory biogenesis factors of mitochondria