PXD047946 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of MIMAS, a multifunctional mega-assembly integrating metabolic and respiratory biogenesis factors of mitochondria |
Description | The mitochondrial inner membrane plays central roles in bioenergetics and metabolism and contains well established membrane protein complexes. Here we report the identification of a megacomplex of the inner membrane, termed mitochondrial multifunctional assembly (MIMAS). Its large size of 3 MDa explains why MIMAS has escaped detection in the analysis of mitochondria so far. MIMAS combines proteins of diverse functions from respiratory assembly to metabolite transport, dehydrogenases and lipid biosynthesis, but not the large established supercomplexes of the respiratory chain, ATP synthase or prohibitin scaffold. MIMAS integrity depends on the non-bilayer phospholipid phosphatidylethanolamine in contrast to respiratory supercomplexes whose stability depends on cardiolipin. Our findings suggest that MIMAS forms a protein-lipid mega-assembly in the mitochondrial inner membrane that integrates respiratory biogenesis and metabolic processes in a multifunctional platform. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:31:28.802.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Robert Hardt |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-19 06:07:52 | ID requested | |
1 | 2024-02-23 03:17:55 | announced | |
⏵ 2 | 2024-10-22 06:31:29 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: innern membrane, yeast,mitochondria, MIMAS, blue native, megacomplex |
Contact List
Thomas Becker |
contact affiliation | Institute for Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, 53115 Bonn, Germany |
contact email | thbecker@uni-bonn.de |
lab head | |
Robert Hardt |
contact affiliation | Institute for Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, 53115 Bonn, Germany |
contact email | rhardt@uni-bonn.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD047946 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047946
- Label: PRIDE project
- Name: Identification of MIMAS, a multifunctional mega-assembly integrating metabolic and respiratory biogenesis factors of mitochondria