PXD047936

PXD047936 is an original dataset announced via ProteomeXchange.

Title	Population context drives cell-to-cell variability in interferon response in epithelial cells
Description	Isogenic cells respond in a heterogeneous manner to interferon. Using a micropatterning approach combined with high-content imaging and spatial analyses, we characterized how the population context (position of a cell with respect to neighboring cells) of epithelial cells affects single cell response to interferons. We identified that cells at the edge of cellular colonies are more responsive than cells embedded within colonies. We determined that this spatial heterogeneity in interferon response resulted from the polarized basolateral interferon receptor distribution, making cells located in the center of cellular colonies less responsive to ectopic interferon stimulation. This was conserved across cell lines and primary cells originated from epithelial tissues. Finally, we demonstrated that this population context-driven cell-to-cell variability influences the outcome of viral infection, as cells embedded within cellular colonies are not protected by interferons and therefore more susceptible to infection. Our data highlights that the behavior of isolated cells does not directly translate to their behavior in a population, placing the population context as one important factor influencing cell-to-cell heterogeneity during interferon response in epithelial cells.
HostingRepository	PRIDE
AnnounceDate	2024-02-07
AnnouncementXML	Submission_2024-02-07_05:15:44.269.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benno Kuropka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-19 03:23:08	ID requested
⏵ 1	2024-02-07 05:15:45	announced

10.1038/s44320-024-00011-2;

Metz-Zumaran C, Uckeley ZM, Doldan P, Muraca F, Keser Y, Lukas P, Kuropka B, K, ü, chenhoff L, Rastgou Talemi S, H, ö, fer T, Freund C, Cavalcanti-Adam EA, Graw F, Stanifer M, Boulant S, The population context is a driver of the heterogeneous response of epithelial cells to interferons. Mol Syst Biol, 20(3):242-275(2024) [pubmed]

submitter keyword: LC-MSMS

Steeve Boulant
contact affiliation	Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida)
contact email	s.boulant@ufl.edu
lab head
Benno Kuropka
contact affiliation	Freie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of Protein Biochemistry, Thielallee 63, D-14195, Berlin, Germany
contact email	kuropka@zedat.fu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD047936

PRIDE project URI

• PRIDE
  1. PXD047936
     1. Label: PRIDE project
     2. Name: Population context drives cell-to-cell variability in interferon response in epithelial cells