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PXD047919

PXD047919 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeubiquitinated peptied quantification of ZmEMB140 by ZmUBP5
DescriptionFlag-EMB140 expressed in HEK293T cells was added to a kernel extract similar to that used in the in vitro stability assay for ZmEMB140 proteins, supplemented with a final concentration of 50mM Mg132. After incubation at room temperature for 1 hour, Flag-EMB140 was precipitated using Flag beads. Following this, SDS-loading buffer was added and the proteins were separated via SDS-PAGE gel electrophoresis
HostingRepositoryiProX
AnnounceDate2023-12-18
AnnouncementXMLSubmission_2023-12-18_19:11:39.508.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKe Li
SpeciesList scientific name: Zea mays; NCBI TaxID: 4577;
ModificationListubiquitination signature dipeptidyl lysine
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-18 19:11:29ID requested
12023-12-18 19:11:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Deubiquitination, maize
Contact List
Zecheng Zuo
contact affiliationJilin University
contact emailzuozhecheng@jlu.edu.cn
lab head
Ke Li
contact affiliationJilin University Collage of Plant Science
contact emaillike8216@mails.jlu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI