PXD047918

PXD047918 is an original dataset announced via ProteomeXchange.

Title	Quantitative analysis of protein ubiquitination levels in wild-type and ubp5 heterozygous mutant maize kernels was performed using DDA (Data-Dependent Acquisition) proteomics
Description	Protein extraction was performed from maize kernels of wild-type and ubp5 heterozygous mutants, collected 30 days after self-pollination. For quantifying ubiquitin levels within the enzymatic digests, a previously established method was adapted with certain modifications. Two milligrams of protein were subjected to alkylation reduction using the aforementioned technique. The reduced proteins were then enzymatically digested employing a trypsin/lys-C protease mix (A41007, Thermo) in 50 mM ammonium bicarbonate overnight. Subsequently, the resulting digest was purified using a MonoSpin C18 spin column (GL Sciences, 5010-21701), eluted with 60% acetonitrile, and rapidly dried under vacuum conditions.Peptide segments were reconstituted in IAP buffer [50 mM MOPS pH 7.2, 10 mM sodium phosphate, 50 mM NaCl], and the isolation of ubiquitin peptides was performed by precipitating them using a K-ɛ-GG-specific antibody (A20303, ABclonal) bound to protein A Dynabeads (10001D, Thermo). Elution of peptides was carried out using 0.15% (vol/vol) TFA, followed by desalting through a MonoSpin C18 spin column and concentration under vacuum using a concentrator.
HostingRepository	iProX
AnnounceDate	2023-12-18
AnnouncementXML	Submission_2023-12-18_19:09:24.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ke Li
SpeciesList	scientific name: Zea mays; NCBI TaxID: 4577;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-18 19:08:59	ID requested
⏵ 1	2023-12-18 19:09:24	announced

Dataset with its publication pending

submitter keyword: Ubiquitination, Kernels, maize

Zecheng Zuo
contact affiliation	Jilin University
contact email	zuozhecheng@jlu.edu.cn
lab head
Ke Li
contact affiliation	Jilin University Collage of Plant Science
contact email	like8216@mails.jlu.edu.cn
dataset submitter

iProX dataset URI