PXD047908

PXD047908 is an original dataset announced via ProteomeXchange.

Title	The tumor suppressor SOCS1 diminishes tolerance to oxidative stress in hepatocellular carcinoma
Description	SOCS1 is a tumor suppressor in hepatocellular carcinoma. Recently we showed that loss of SOCS1 in hepatocytes promotes NRF2 activation. Here we investigated how SOCS1 expression affected oxidative stress response in HCC cells. Murine Hepa1-6 cells expressing SOCS1 (Hepa-SOCS1) or control vector (Hepa-Vector) were treated with cisplatin or tert-butyl hydroperoxide (t-BHP). Induction of NRF2 and its target genes, oxidative stress, lipid peroxidation, cell survival and cellular proteome profiles were evaluated. NRF2 induction was significantly reduced in Hepa-SOCS1 cells. The gene and protein expression of NRF2 targets were differentially induced in Hepa-Vector cells but markedly suppressed in Hepa-SOCS1 cells. Hepa-SOCS1 cells displayed increased induction of reactive oxygen species (ROS) but reduced lipid peroxidation. Nonetheless, Hepa-SOCS1 cells treated with cisplatin or t-BHP showed reduced survival. GCLC, poorly induced in Hepa-SOCS1 cells, showed a strong positive correlation with NFE2L2 and an inverse correlation with SOCS1 in the TCGA-LIHC transcriptomic data. Proteomic analysis of Hepa-Vector and Hepa-SOCS1 cells revealed that SOCS1 differentially modulated many proteins involved in diverse molecular pathways including those implicated in mitochondrial ROS generation and detoxification by peroxiredoxin and thioredoxin systems. Our findings indicate that maintaining sensitivity to oxidative stress is an important tumor suppression mechanism of SOCS1 in HCC.
HostingRepository	PRIDE
AnnounceDate	2024-05-22
AnnouncementXML	Submission_2024-05-22_04:57:08.177.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jean-Francois Lucier
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Bruker Daltonics instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-18 10:04:53	ID requested
⏵ 1	2024-05-22 04:57:09	announced

Ilangumaran S, Gui Y, Shukla A, Ramanathan S, SOCS1 expression in cancer cells: potential roles in promoting antitumor immunity. Front Immunol, 15():1362224(2024) [pubmed]

10.3389/fimmu.2024.1362224;

submitter keyword: Hepatocellular carcinoma

SOCS1

oxidative stress

cisplatin

tert-butyl hydroperoxide

mass spectrometry

Subburaj Ilangumaran
contact affiliation	Department of Immunology and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Québec J1H 5N4, Canada
contact email	Subburaj.Ilangumaran@USherbrooke.ca
lab head
Jean-Francois Lucier
contact affiliation	Universite de Sherbrooke
contact email	jean-francois.lucier@usherbrooke.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047908
     1. Label: PRIDE project
     2. Name: The tumor suppressor SOCS1 diminishes tolerance to oxidative stress in hepatocellular carcinoma