PXD047889

PXD047889 is an original dataset announced via ProteomeXchange.

Title	Data-Independent Acquisition proteomics of protein extracted from kernels collected at fifteen and thirty days post self-pollination of the ubp5 mutant heterozygous (smd/+) and wild-type specimens
Description	protein extraction was performed from kernels collected at fifteen and thirty days post self-pollination of the ubp5 mutant heterozygous (smd/+) and wild-type specimens using phenol. For quantifying protein expression levels, 500μg of dissolved protein underwent reduction by 50 mM DTT (3483-12-3, Sigma-Aldrich) at 37°C for 1 hour. Subsequently, 150 mM iodoacetamide (144-48-9, Sigma-Aldrich) was added to modify the reduced sulfhydryl groups. Following a 30-minute incubation period in darkness at room temperature, the protein sample underwent buffer exchange to 50 mM ammonium bicarbonate (A6141, Sigma-Aldrich) through an ultrafiltration column (UFC501096, Millipore). Enzymatic digestion was carried out using trypsin (V5111, Promega), and the resulting digest underwent purification using C18 Spin Tips (84850, Thermo) followed by elution with 60% acetonitrile and rapid drying under vacuum conditions.
HostingRepository	iProX
AnnounceDate	2023-12-17
AnnouncementXML	Submission_2023-12-17_20:29:00.091.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li Zhang
SpeciesList	scientific name: Zea mays; NCBI TaxID: 4577;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-17 20:28:29	ID requested
⏵ 1	2023-12-17 20:29:00	announced

Dataset with its publication pending

submitter keyword: Data-Independent Acquisition, maize, kernel

Zecheng Zuo
contact affiliation	Jilin University
contact email	zuozhecheng@jlu.edu.cn
lab head
Li Zhang
contact affiliation	Jilin University
contact email	Zhang_li18@mails.jlu.edu.cn
dataset submitter

iProX dataset URI