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PXD047870

PXD047870 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePRM validation of phosphoproteomic and proteomic analysis of early epileptogenesis in mice at 4 and 24 h post-status epilepticus
DescriptionThe phosphorylation-based signalling and protein changes occurring in the early phases after a pathophysiological insult, like status epilepticus (SE), have not been detailed. In a companion project, the hippocampi of mice treated with pilocarpine and diazepam were examined by tandem mass tag (TMT11plex) mass spectrometry at 4 and 24 h post-status epilepticus (PXD038241). In the accompanying article, the results implicated posttranscriptional regulatory proteins as early targets of increased phosphorylation. Also, the major targets of decreased phosphorylation at 4 h and 24 h were a subset of post synaptic density scaffold proteins, ion channels and neurotransmitter receptors. Here, the earlier work is repeated on protein and phosphorylation site targets representative of the important SE-dependent changes using parallel reaction monitoring (PRM), supporting the main findings.
HostingRepositoryPRIDE
AnnounceDate2024-04-30
AnnouncementXMLSubmission_2024-04-30_06:15:08.534.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD047870
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMark Graham
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-16 18:21:45ID requested
12024-02-21 21:00:20announced
22024-04-30 06:15:09announced2024-04-30: Updated project metadata.
Publication List
10.1016/j.isci.2024.109534;
10.6019/PXD047870;
Hurtado Silva M, van Waardenberg AJ, Mostafa A, Schoch S, Dietrich D, Graham ME, Multiomics of early epileptogenesis in mice reveals phosphorylation and dephosphorylation-directed growth and synaptic weakening. iScience, 27(4):109534(2024) [pubmed]
Keyword List
submitter keyword: Epileptogenesis, Phospho-signalling, proteomics, epilepsy, hippocampus, synaptic weakening, signalling, phosphorylation, mouse, parallel reaction monitoring, synapse growth
Contact List
Mark Evan Graham
contact affiliationSynapse Proteomics, Children's Medical Research Insitute
contact emailmgraham@cmri.org.au
lab head
Mark Graham
contact affiliationChildren's Medical Research Institute
contact emailmgraham@cmri.org.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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