PXD047870 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PRM validation of phosphoproteomic and proteomic analysis of early epileptogenesis in mice at 4 and 24 h post-status epilepticus |
Description | The phosphorylation-based signalling and protein changes occurring in the early phases after a pathophysiological insult, like status epilepticus (SE), have not been detailed. In a companion project, the hippocampi of mice treated with pilocarpine and diazepam were examined by tandem mass tag (TMT11plex) mass spectrometry at 4 and 24 h post-status epilepticus (PXD038241). In the accompanying article, the results implicated posttranscriptional regulatory proteins as early targets of increased phosphorylation. Also, the major targets of decreased phosphorylation at 4 h and 24 h were a subset of post synaptic density scaffold proteins, ion channels and neurotransmitter receptors. Here, the earlier work is repeated on protein and phosphorylation site targets representative of the important SE-dependent changes using parallel reaction monitoring (PRM), supporting the main findings. |
HostingRepository | PRIDE |
AnnounceDate | 2024-04-30 |
AnnouncementXML | Submission_2024-04-30_06:15:08.534.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD047870 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Mark Graham |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-16 18:21:45 | ID requested | |
1 | 2024-02-21 21:00:20 | announced | |
⏵ 2 | 2024-04-30 06:15:09 | announced | 2024-04-30: Updated project metadata. |
Publication List
10.1016/j.isci.2024.109534; |
10.6019/PXD047870; |
Hurtado Silva M, van Waardenberg AJ, Mostafa A, Schoch S, Dietrich D, Graham ME, Multiomics of early epileptogenesis in mice reveals phosphorylation and dephosphorylation-directed growth and synaptic weakening. iScience, 27(4):109534(2024) [pubmed] |
Keyword List
submitter keyword: Epileptogenesis, Phospho-signalling, proteomics, epilepsy, hippocampus, synaptic weakening, signalling, phosphorylation, mouse, parallel reaction monitoring, synapse growth |
Contact List
Mark Evan Graham |
contact affiliation | Synapse Proteomics, Children's Medical Research Insitute |
contact email | mgraham@cmri.org.au |
lab head | |
Mark Graham |
contact affiliation | Children's Medical Research Institute |
contact email | mgraham@cmri.org.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047870
- Label: PRIDE project
- Name: PRM validation of phosphoproteomic and proteomic analysis of early epileptogenesis in mice at 4 and 24 h post-status epilepticus