PXD047868

PXD047868 is an original dataset announced via ProteomeXchange.

Title	p53 is a suppressor of the carcinoma-associated transcription factor FOXQ1
Description	FOXQ1 transcription factors is highly induced in several types of carcinomas where it promotes tumour growth and metastasis, however, the molecular mechanisms leading to FOXQ1 deregulation in cancer are incompletely understood. Here, we used a CRISPR/Cas9-based genomic locus proteomics (GLoPro) to discover transcriptional regulators of FOXQ1 and identified the tumour suppressor p53 as a transcriptional repressor of FOXQ1 expression. ChIP-qPCR as well as complementary gain and loss-of-function assays in model cell lines indicated that p53 has multiple binding sites close to the transcription start site of the FOXQ1 promoter, and that it suppresses FOXQ1 expression in various cell types. Consistently, pharmacological activation of p53 using nutlin-3 or doxorubicin reduced FOXQ1 mRNA and protein levels in colorectal cancer cell lines harboring wild type p53. In conclusion, these results suggest that mutational loss-of-function of p53, a hallmark feature of many types of cancer, de-represses FOXQ1, which accelerates tumour progression.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:39:07.194.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047868
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Giulia Pizzolato
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-16 08:23:56	ID requested
1	2024-05-13 07:33:01	announced
⏵ 2	2024-10-22 06:39:07	announced	2024-10-22: Updated project metadata.

10.1016/J.JBC.2024.107126;

10.6019/PXD047868;

submitter keyword: FOXQ1, P53, GLoPro, Cancer, Transcription factor

Dr.Stefan Koch
contact affiliation	Wallenberg Centre for Molecular Medicine (WCMM), Linköping University, Linköping, Sweden; Department of Biomedical and Clinical Sciences (BKV), Linköping University, Linköping, Sweden.
contact email	Stefan.koch@liu.se
lab head
Giulia Pizzolato
contact affiliation	Center for Molecular Medicine Cologne (CMMC) University of Cologne
contact email	giulia.pizzolato@uk-koeln.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047868
     1. Label: PRIDE project
     2. Name: p53 is a suppressor of the carcinoma-associated transcription factor FOXQ1