PXD047862 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mycobacterium abscessus Extracellular Vesicles Increase Mycobacterial Resistance to Clarithromycin in vitro |
| Description | Non-tuberculous Mycobacteria (NTM) are a group of emerging bacterial pathogens that have been identified in cystic fibrosis (CF) patients with microbial lung infections. The treatment of NTM infection in CF patients is challenging due to the natural resistance of NTM species to many antibiotics. Mycobacterium abscessus (M. abscessus) is one of the most common NTM strains found in the airway of CF patients. In our current study, we characterized the extracellular vesicles (EVs) released by drug-sensitive M. abscessus untreated or treated with clarithromycin, one of the well-known anti-NTM drugs. Our data show that clarithromycin treatment increases mycobacterial protein trafficking into EVs as well as the secretion of EVs in M. abscessus culture. Additionally, EVs released by clarithromycin-treated M. abscessus increase M. abscessus resistance to clarithromycin when compared to EVs from untreated M. abscessus. EV proteomic analysis further indicates that EVs released by clarithromycin-treated M. abscessus carry an increased level of 50S ribosomal subunits, the target of clarithromycin. Taken together, our results suggest that mycobacterial EVs play an important role in increasing M. abscessus resistance to clarithromycin treatment. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-05-06 |
| AnnouncementXML | Submission_2025-05-06_12:30:41.717.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Steven Hartson |
| SpeciesList | scientific name: Mycobacterium abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543); NCBI TaxID: 561007; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-12-15 14:21:40 | ID requested | |
| ⏵ 1 | 2025-05-06 12:30:42 | announced | |
Publication List
| Vermeire CA, Tan X, Liang Y, Kotey SK, Rogers J, Hartson SD, Liu L, Cheng Y, Mycobacterium abscessus extracellular vesicles increase mycobacterial resistance to clarithromycin in vitro. Proteomics, 24(10):e2300332(2024) [pubmed] |
| 10.1002/pmic.202300332; |
Keyword List
| submitter keyword: Extracellular Vesicles, Clarithromycin,Mycobacterium abscessus |
Contact List
| Dr Yong Cheng |
|---|
| contact affiliation | Department of Biochemistry and Molecular Biology Oklahoma State University Stillwater, OK 74078 USA |
| contact email | ycheng@okstate.edu |
| lab head | |
| Steven Hartson |
|---|
| contact affiliation | Oklahoma State University |
| contact email | hartson.steve@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD047862 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047862
- Label: PRIDE project
- Name: Mycobacterium abscessus Extracellular Vesicles Increase Mycobacterial Resistance to Clarithromycin in vitro
to receive all new ProteomeXchange dataset release announcements!
