PXD047860
PXD047860 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Exploration of adult mouse Single Cardiomyocyte Proteome using Label-Free LC-MS |
| Description | Cardiac myocyte heterogeneity has been acknowledged based on ion channel expression, cellular electrophysiology and through transcriptomics, but the exploration of individual cardiomyocytes at the board proteome-wide level has remained challenging. We developed an entire analytical pipeline to assess the cell heterogeneity of adult primary cardiomyocytes isolated from mouse hearts. Over 700 single cell proteomes were isolated from 4 mice hearts. Each single cell image was captured by the cellenONE sorter interface and evaluated for morphological features of apoptotic cells, i.e.: hypercontracted cell shape. This imaging analysis allowed us to retain 270 rod-shaped cardiac cells for downstream single cell proteome sample processing and analysis on Bruker timsTOF SCP mass spectrometer. Final dataset UMAP dimension reduction and clustering analysis yielded 3 cardiomyocyte cell sub-populations that displayed differential: i) expression of Myh7 and Myh7b, ii) carbon metabolism signatures and iii) distribution of 7 proteins involved in calcium signaling. Single cardiomyocyte protemes demonstrated potential differences related to the metabolism and calcium ion handling between those sub-populations and could bring new insights for cardiology studies on mice. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-03-20 |
| AnnouncementXML | Submission_2024-03-20_16:21:01.505.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Aleksandra Binek |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF SCP |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-12-15 11:40:19 | ID requested | |
| ⏵ 1 | 2024-03-20 16:21:01 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Single cell proteomics, mass spectrometry, cardiomyocyte, heart, myosin, mouse |
Contact List
| Jennifer Van Eyk | |
|---|---|
| contact affiliation | Cedars Sinai Medical Center |
| contact email | jennifer.vaneyk@cshs.org |
| lab head | |
| Aleksandra Binek | |
| contact affiliation | Cedars Sinai Medical Center |
| contact email | bineka@cshs.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093672/ |
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