PXD047849

PXD047849 is an original dataset announced via ProteomeXchange.

Title	Comparative_Interaction_Proteomics_GFP-tagged_MAGEC2_HEK293T
Description	293T (Human embryonic kidney cells-ATCC CRL-3216) cells were plated at 500,000 cells per 10 cm dish and grown for 48 hours. Cells were then transfected with GFP-MAGEC1 WT or GFP-MAGEC1 L318D in a 3:1 ratio of PEI (polyethylenimine) to DNA. Cells were harvested after 48 hours and lysed in 50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100, protease inhibitor (cOmplete™, Mini, EDTA-free (Roche)). Cells were incubated for 25 minutes on ice and then sonicated for 5 minutes at 4°C. Cells were centrifuged at 14,000 rpm for 20 minutes at 4 °C. 500 ug lysate was then added to GFP-Trap® Agarose resin (ChromoTek) and samples were incubated rotating at 4°C for 2 hours. Following washing of the beads with 50 mM Tris pH 7.5, 150 mM NaCl, proteins were eluted by the addition of 2x Laemmli buffer and boiled for 10 minutes at 95 °C. Samples were spun down and the supernatants were sent for mass spectrometry analysis as described below. Experiments were conducted in triplicate.
HostingRepository	PRIDE
AnnounceDate	2024-02-20
AnnouncementXML	Submission_2024-02-20_03:28:21.306.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-15 01:31:44	ID requested
⏵ 1	2024-02-20 03:28:21	announced

Dataset with its publication pending

submitter keyword: HEK293T cells,MAGEC2, Proteomics

Sagar Bhogaraju
contact affiliation	EMBL Grenoble
contact email	bhogaraju@embl.fr
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD047849

PRIDE project URI

• PRIDE
  1. PXD047849
     1. Label: PRIDE project
     2. Name: Comparative_Interaction_Proteomics_GFP-tagged_MAGEC2_HEK293T