PXD047832

PXD047832 is an original dataset announced via ProteomeXchange.

Title	Green leaf volatiles co-opt proteins involved in molecular pattern signaling in plant cells
Description	The green leaf volatiles (GLVs) Z-3-hexen-1-ol (Z3-HOL) and Z-3-hexenyl acetate (Z3-HAC) are airborne infochemicals released from damaged plant tissues that induce defenses and developmental responses in receiver plants, but little is known about their mechanism of action. We found that Z3-HOL and Z3-HAC induce similar but distinctive physiological and signaling responses in tomato seedlings and cell cultures. In seedlings, Z3-HAC showed a stronger root growth inhibition effect than Z3-HOL. In cell cultures, the two GLVs induced distinct changes in MAP kinase activity and proton fluxes as well as rapid and massive changes in the phosphorylation status of proteins within five minutes. Many of these phosphoproteins are involved in reprogramming the proteome from cellular homeostasis to stress and include pattern recognition receptors, a receptor-like cytoplasmic kinase, MAPK cascade components, calcium signaling proteins, and transcriptional regulators. These are well-known components of damage-associated molecular pattern (DAMP) signaling pathways. These rapid changes in the phosphoproteome may underly the activation of defense and developmental responses to GLVs. Our data provide further evidence that GLVs function like DAMPs and indicate that GLVs coopt DAMP signaling pathways.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:31:08.775.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sasimonthakan Tanarsuwongkul
SpeciesList	scientific name: Solanum peruvianum; NCBI TaxID: 4082;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-14 07:38:54	ID requested
1	2024-02-19 08:10:49	announced
⏵ 2	2024-10-22 06:31:09	announced	2024-10-22: Updated project metadata.

Tanarsuwongkul S, Fisher KW, Mullis BT, Negi H, Roberts J, Tomlin F, Wang Q, Stratmann JW, Green leaf volatiles co-opt proteins involved in molecular pattern signalling in plant cells. Plant Cell Environ, 47(3):928-946(2024) [pubmed]

10.1111/pce.14795;

submitter keyword: MAP kinase (MAPK), DAMP, systemin,green leaf volatiles (GLVs), molecular pattern, volatile organic compounds (VOCs), phosphoproteomics, pattern recognition receptor

Johannes Stratmann
contact affiliation	Department of Biological Sciences College of Arts and Sciences University of South Carolina
contact email	johstrat@biol.sc.edu
lab head
Sasimonthakan Tanarsuwongkul
contact affiliation	Department of Chemistry and Biochemistry, University of South Carolina
contact email	tanarsus@mailbox.sc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047832
     1. Label: PRIDE project
     2. Name: Green leaf volatiles co-opt proteins involved in molecular pattern signaling in plant cells