PXD047821

PXD047821 is an original dataset announced via ProteomeXchange.

Title	Affinity-purification of V5-tagged VZV ORFs in SK-N-BE2 cells
Description	The alpha-herpesvirus Varicella-Zoster Virus (VZV), the causative agent of chicken pox, infects most humans worldwide and remains latent in peripheral neuronal cells for life. Its reactivation triggers herpes zoster and sporadic disseminated infection, in particular in the central nervous system (CNS) where it is associated with severe pathologies. VZV replication is efficiently controlled with antivirals, however the emergence of treatment-resistant VZV variants constitutes an increasing healthcare issue, calling for in-depth analysis of molecular VZV-host interactions to foster general knowledge and promote identification of novel therapeutic targets. Here we conducted a mass spectrometry-based survey in neuronal SK-N-BE2 cells, combining proteome analysis of VZV-infected cells with systematic characterization of VZV proteins’ functions through identifying their individual host partners and effects on the host proteome. To generate this specific dataset, we used AP-MS and a Bayesian modelling approach to characterize the VZV-host interactome. We collectively identified 1184 interactions between 56 viral and 900 human proteins.
HostingRepository	PRIDE
AnnounceDate	2025-07-15
AnnouncementXML	Submission_2025-07-14_17:59:37.623.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginie Girault
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human alphaherpesvirus 3; NCBI TaxID: 10335;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-14 03:03:54	ID requested
⏵ 1	2025-07-14 17:59:38	announced

10.1038/S41564-025-02068-7;

submitter keyword: VZV, SK-N-BE2, affinity-purification, VZV ORF

Andreas Pichlmair
contact affiliation	Institute of Virology, Technical University of Munich, Munich, Germany.
contact email	andreas.pichlmair@tum.de
lab head
Virginie Girault
contact affiliation	Institute of Virology - Technical University Munich
contact email	v.girault@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD047821

PRIDE project URI

• PRIDE
  1. PXD047821
     1. Label: PRIDE project
     2. Name: Affinity-purification of V5-tagged VZV ORFs in SK-N-BE2 cells