PXD047799

PXD047799 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Decrypting the molecular basis of cellular drug phenotypes by dose-resolved expression proteomics
Description	Proteomics is making important contributions to drug discovery - from target deconvolution to mechanism of action (MoA) elucidation and the identification of biomarkers of drug response. Here, we introduce decryptE, a proteome-wide approach that measures the full dose-response characteristics of drug-induced protein expression changes which informs cellular drug MoA. Assaying 144 clinical drugs and research compounds against 8,000 proteins resulted in >1 million dose-response curves that can be interactively explored online in ProteomicsDB and a custom-built ShinyApp. Analysis of the collective data provided molecular explanations for known phenotypic drug effects and uncovered novel aspects of the MoAs of human medicines. Most notable, HDAC inhibitors potently and strongly down-regulated the T-cell receptor complex resulting in impaired human T-cell activation in-vitro and ex-vivo. This not only offers a rational explanation for the efficacy of HDAC inhibitors in certain lymphomas and autoimmune diseases but also their poor performance in treating solid tumors.
HostingRepository	MassIVE
AnnounceDate	2024-03-04
AnnouncementXML	Submission_2024-03-04_10:10:48.975.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Eckert
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Escherichia coli; common name: E. coli; NCBI TaxID: 562; scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287; scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl; Acetyl; Methyl; Dimethyl
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-13 12:34:55	ID requested
⏵ 1	2024-03-04 10:10:49	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: FAIMS, micro-flow liquid chromatography, drug screen, drugs, decryptE, mode of action, dose-response, protein expression profiles, primary T cells, HDAC inhibitors, HDAC, proteomics

submitter keyword: FAIMS, micro-flow liquid chromatography, drug screen, drugs, decryptE, mode of action, dose-response, protein expression profiles, primary T cells, HDAC inhibitors, HDAC, proteomics

Contact List

Bernhard Kuster
contact affiliation	Technical University of Munich (TUM)
contact email	kuster@tum.de
lab head
Stephan Eckert
contact affiliation	Technical University Munich (TUM)
contact email	stephan.eckert@tum.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093659/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093659/