PXD047788

PXD047788 is an original dataset announced via ProteomeXchange.

Title	Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neurons
Description	Large-scale genomic screenings greatly advanced our understanding of the genetic basis of schizophrenia. However, how this translates into neurobiological functioning is not clear. Therefore, we aimed to characterize the function of robust susceptibility gene zinc finger protein 804A (ZNF804A), using a model system of relevant cell type and neurodevelopmental timepoint. Based on previous findings, we hypothesized a role for ZNF804A in local protein translation. CRISPR/Cas9-mediated mutation of the risk gene led to increased synapse formation in human induced pluripotent stem cell (hiPSC)-derived developing glutamatergic forebrain neurons as assessed by high-content confocal imaging. Further, mass spectrometry analysis showed an increased recruitment of ribosomes to mutation neurites. Surface sensing of translation (SUnSET) screening complemented this by demonstrating an increased efficiency of local protein synthesis. Overall, these results introduce a novel cellular function for ZNF804A in a model system relevant to schizophrenia, uncovering some aspects of its neurobiology. Ultimately, findings of this study may aid to identify common mechanisms of susceptibility genes.
HostingRepository	PRIDE
AnnounceDate	2026-04-01
AnnouncementXML	Submission_2026-03-31_16:03:57.733.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maximilian Wulf
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-13 03:49:53	ID requested
⏵ 1	2026-03-31 16:03:58	announced

Dataset with its publication pending

submitter keyword: ribosomes, compartment seperation assay,schizophrenia, data-independent acquisition, synaptogenesis

Deepak P. Srivastava
contact affiliation	Department of Basic and Clinical Neuroscience, The Maurice Wohl Clinical Neuroscience Institute, Institute of Psychiatry, Psychology, & Neuroscience, King’s College London, London, UK
contact email	deepak.srivastava@kcl.ac.uk
lab head
Maximilian Wulf
contact affiliation	Medizinisches Proteom-Center, Ruhr-University Bochum
contact email	maximilian.wulf@ruhr-uni-bochum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD047788

PRIDE project URI

• PRIDE
  1. PXD047788
     1. Label: PRIDE project
     2. Name: Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neurons